关键词: Embryo selection IMSI MSOME meiotic spindle polarization microscope time-lapse monitoring zona pellucida birefringence

Mesh : Embryo, Mammalian / cytology metabolism Embryonic Development Female Fertilization in Vitro Germ Cells / cytology metabolism Humans Male Microscopy / methods Molecular Imaging / methods Pregnancy Reproductive Techniques, Assisted Time-Lapse Imaging / methods

来  源:   DOI:10.1111/jmi.12573   PDF(Sci-hub)

Abstract:
Optimizing the efficiency of the in vitro fertilization procedure by improving pregnancy rates and reducing the risks of multiple pregnancies simultaneously are the primary goals of the current assisted reproductive technology program. With the move to single embryo transfers, the need for more cost-effective and noninvasive methods for embryo selection prior to transfer is paramount. These aims require advancement in a more acquire gametes/embryo testing and selection procedures using high-tech devices. Therefore, the aim of the present review is to evaluate the efficacy of noninvasive imaging systems in the current literatures, focusing on the potential clinical application in infertile patients undergoing assisted reproductive technology treatments. In this regards, three advanced imaging systems of motile sperm organelle morphology examination, polarization microscopy and time-lapse monitoring for the best selection of the gametes and preimplantation embryos are introduced in full.
摘要:
通过提高妊娠率和同时降低多胎妊娠的风险来优化体外受精程序的效率是当前辅助生殖技术计划的主要目标。随着单胚胎移植的发展,对于移植前胚胎选择的更具成本效益的非侵入性方法的需求至关重要.这些目标需要在使用高科技设备的更多获取配子/胚胎测试和选择程序中取得进步。因此,本综述的目的是评估现有文献中的无创成像系统的疗效,重点介绍在接受辅助生殖技术治疗的不孕患者中的潜在临床应用。在这方面,三种先进的能动精子细胞器形态学检查成像系统,充分介绍了偏振显微镜和延时监测,以最佳选择配子和植入前胚胎。
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