Abstract：
OBJECTIVE: The most common single nucleotide polymorphism in the IGFBP3 promoter region occurs at position -202. This polymorphic variation occurs frequently and may influence growth hormone responsiveness and somatic growth. However, the effects of IGFBP3 promoter polymorphism on growth in children are unknown.
METHODS: Restriction fragment length polymorphism-based genotyping of the -202 single nucleotide polymorphism was performed in 146 Korean girls aged between 15 and 16 years, who were selected randomly from the Seoul School Health Promotion Center. The participants were divided into 3 groups (tall, medium, and short) according to the height percentile established from normal reference values for Korean children. The serum levels of insulin-like growth factor I (IGF-I) and IGF-binding protein-3 (IGFBP-3) were then compared according to genotype.
RESULTS: The genotype distribution in the participants was 79 AA (54.1%), 60 AC (41.1%), and 7 CC (4.8%). The C allele frequency at the -202 IGFBP3 position was 25.4% in this group. The mean serum IGFBP-3 concentration in girls with the AA genotype was higher than that in girls with the AC genotype in the medium (P=0.047) and short (P=0.035) groups, respectively. There was no difference in the IGF-I to IGFBP-3 molar ratio between the AA and AC genotype groups (P=0.161).
CONCLUSIONS: In conclusion, the -202 polymorphism in the IGFBP3 promoter region is assumed to affect the serum concentration of IGFBP-3 in children as well as in adults. However, it is unclear whether this affects physical development according to the concentration of IGFBP-3.
METHODS: Restriction fragment length polymorphism-based genotyping of the -202 single nucleotide polymorphism was performed in 146 Korean girls aged between 15 and 16 years, who were selected randomly from the Seoul School Health Promotion Center. The participants were divided into 3 groups (tall, medium, and short) according to the height percentile established from normal reference values for Korean children. The serum levels of insulin-like growth factor I (IGF-I) and IGF-binding protein-3 (IGFBP-3) were then compared according to genotype.
RESULTS: The genotype distribution in the participants was 79 AA (54.1%), 60 AC (41.1%), and 7 CC (4.8%). The C allele frequency at the -202 IGFBP3 position was 25.4% in this group. The mean serum IGFBP-3 concentration in girls with the AA genotype was higher than that in girls with the AC genotype in the medium (P=0.047) and short (P=0.035) groups, respectively. There was no difference in the IGF-I to IGFBP-3 molar ratio between the AA and AC genotype groups (P=0.161).
CONCLUSIONS: In conclusion, the -202 polymorphism in the IGFBP3 promoter region is assumed to affect the serum concentration of IGFBP-3 in children as well as in adults. However, it is unclear whether this affects physical development according to the concentration of IGFBP-3.
摘要：
目的：IGFBP3启动子区最常见的单核苷酸多态性发生在-202位。这种多态性变异经常发生，可能会影响生长激素反应性和体细胞生长。然而,IGFBP3启动子多态性对儿童生长的影响尚不清楚。
方法：对146名年龄在15至16岁之间的韩国女孩进行了基于限制性片段长度多态性的-202单核苷酸多态性基因分型，他们是从首尔学校健康促进中心随机挑选的。参与者分为3组(高个子，中等,和短）根据韩国儿童的正常参考值确定的身高百分位数。然后根据基因型比较胰岛素样生长因子I（IGF-I）和IGF结合蛋白3（IGFBP-3）的血清水平。
结果：参与者的基因型分布为79AA（54.1％），60交流电（41.1%），和7CC（4.8%）。在该组中,在-202IGFBP3位置处的C等位基因频率为25.4%。在中(P=0.047)和短(P=0.035)组,AA基因型女孩的平均血清IGFBP-3浓度高于AC基因型女孩,分别。AA和AC基因型组之间的IGF-I与IGFBP-3摩尔比没有差异（P=0.161）。
结论：结论：IGFBP3启动子区域的-202多态性被认为会影响儿童和成人的血清IGFBP-3浓度.然而,尚不清楚这是否根据IGFBP-3的浓度影响身体发育。
方法：对146名年龄在15至16岁之间的韩国女孩进行了基于限制性片段长度多态性的-202单核苷酸多态性基因分型，他们是从首尔学校健康促进中心随机挑选的。参与者分为3组(高个子，中等,和短）根据韩国儿童的正常参考值确定的身高百分位数。然后根据基因型比较胰岛素样生长因子I（IGF-I）和IGF结合蛋白3（IGFBP-3）的血清水平。
结果：参与者的基因型分布为79AA（54.1％），60交流电（41.1%），和7CC（4.8%）。在该组中,在-202IGFBP3位置处的C等位基因频率为25.4%。在中(P=0.047)和短(P=0.035)组,AA基因型女孩的平均血清IGFBP-3浓度高于AC基因型女孩,分别。AA和AC基因型组之间的IGF-I与IGFBP-3摩尔比没有差异（P=0.161）。
结论：结论：IGFBP3启动子区域的-202多态性被认为会影响儿童和成人的血清IGFBP-3浓度.然而,尚不清楚这是否根据IGFBP-3的浓度影响身体发育。
