关键词: active oxygen species cat liver fluke Opisthorchis felineus excretory-secretory product opisthorchiasis thioredoxin peroxidase

Mesh : Animals Antibodies / chemistry isolation & purification Bile Ducts / enzymology parasitology Blotting, Western Cloning, Molecular Disease Models, Animal Escherichia coli / genetics metabolism Fishes / parasitology Gene Expression Helminth Proteins / agonists genetics metabolism Hydrogen Peroxide / pharmacology Immunohistochemistry Mesocricetus / parasitology Opisthorchiasis / enzymology parasitology Opisthorchis / drug effects enzymology genetics isolation & purification Oxidative Stress Peroxiredoxins / genetics metabolism Rabbits Recombinant Proteins / administration & dosage genetics immunology

来  源:   DOI:10.1007/s10517-017-3710-5

Abstract:
Mechanisms of thioredoxin peroxidase secretion by Opisthorchis felineus were studied in vivo and in vitro. Specific antibodies were obtained and used for western blotting and immunohistochemical detection in Syrian hamster model of opisthorchiasis. Secreted thioredoxin peroxidase protein was accumulated in the worm incubation medium under conditions of oxidative stress and in bile duct cells of hamsters with chronic opisthorchiasis.
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