PDF(Sci-hub)
Abstract：
BACKGROUND: The purpose of our study was to determine prospectively relationships between plasma mitochondrial deoxyribonucleic acid (mtDNA) concentration and clinical outcome in patients with intra-abdominal infections (IAIs) induced by severe abdominal trauma.
METHODS: The DNA was isolated from serum samples taken from patients with IAIs at hospital days zero, one, and two. Plasma mtDNA concentration was assessed by real-time polymerase chain reaction (PCR). The study population\'s clinical and laboratory data were analyzed.
RESULTS: The mtDNA damage-associated molecular patterns were expressed as a PCR threshold cycle number using four selected sequences. The patients with IAIs had significant higher plasma mtDNA than healthy control subjects. Patients with IAIs with sepsis apparently had elevated mtDNA levels compared with non-septic patients with IAIs (30.9 ± 2.0 vs. 28.7 ± 2.4; 33.3 ± 2.6 vs. 28.9 ± 2.4; 32.9 ± 1.6 vs. 31.2 ± 2.2; 33.1 ± 3.6 vs. 28.1 ± 2.2, respectively). Patients with IAIs in whom multiple organ dysfunction syndrome (MODS) developed also had increased mtDNA concentration compared with those who did not (31.0 ± 1.8 vs. 27.9 ± 1.8; 32.9 ± 2.4 vs. 27.8 ± 1.7; 32.9 ± 1.5 vs. 29.8 ± 1.7; 32.0 ± 3.8 vs. 27.1 ± 2.1, respectively). Baseline mtDNA concentration had high effectiveness in predicting death for patients with IAIs who had severe trauma using receiver operating characteristic analysis. Furthermore, serum mtDNA levels on admission correlated with the lactate concentration, but no significant correlations were found between mtDNA levels and levels of white blood cells, C-reactive protein, and procalcitonin.
CONCLUSIONS: Plasma mtDNA was associated with the occurrence of sepsis, MODS, and death in patients with IAIs caused by severe abdominal trauma.
METHODS: The DNA was isolated from serum samples taken from patients with IAIs at hospital days zero, one, and two. Plasma mtDNA concentration was assessed by real-time polymerase chain reaction (PCR). The study population\'s clinical and laboratory data were analyzed.
RESULTS: The mtDNA damage-associated molecular patterns were expressed as a PCR threshold cycle number using four selected sequences. The patients with IAIs had significant higher plasma mtDNA than healthy control subjects. Patients with IAIs with sepsis apparently had elevated mtDNA levels compared with non-septic patients with IAIs (30.9 ± 2.0 vs. 28.7 ± 2.4; 33.3 ± 2.6 vs. 28.9 ± 2.4; 32.9 ± 1.6 vs. 31.2 ± 2.2; 33.1 ± 3.6 vs. 28.1 ± 2.2, respectively). Patients with IAIs in whom multiple organ dysfunction syndrome (MODS) developed also had increased mtDNA concentration compared with those who did not (31.0 ± 1.8 vs. 27.9 ± 1.8; 32.9 ± 2.4 vs. 27.8 ± 1.7; 32.9 ± 1.5 vs. 29.8 ± 1.7; 32.0 ± 3.8 vs. 27.1 ± 2.1, respectively). Baseline mtDNA concentration had high effectiveness in predicting death for patients with IAIs who had severe trauma using receiver operating characteristic analysis. Furthermore, serum mtDNA levels on admission correlated with the lactate concentration, but no significant correlations were found between mtDNA levels and levels of white blood cells, C-reactive protein, and procalcitonin.
CONCLUSIONS: Plasma mtDNA was associated with the occurrence of sepsis, MODS, and death in patients with IAIs caused by severe abdominal trauma.
摘要：
背景：我们的研究目的是确定严重腹部创伤引起的腹腔内感染（IAI）患者血浆线粒体脱氧核糖核酸（mtDNA）浓度与临床预后之间的前瞻性关系。
方法：在医院第0天从IAIs患者的血清样本中分离DNA，一,还有两个.通过实时聚合酶链反应(PCR)评估血浆mtDNA浓度。分析研究人群的临床和实验室数据。
结果：使用四个选择的序列，将mtDNA损伤相关的分子模式表示为PCR阈值循环数。IAI患者的血浆mtDNA明显高于健康对照组。与具有IAIs的非脓毒症患者相比，具有IAIs的IAIs患者的mtDNA水平明显升高（30.9±2.0vs.28.7±2.4;33.3±2.6vs.28.9±2.4;32.9±1.6vs.31.2±2.2;33.1±3.6vs.分别为28.1±2.2)。与未发生多器官功能障碍综合征（MODS）的IAIs患者相比，其mtDNA浓度也增加了（31.0±1.8vs.27.9±1.8;32.9±2.4vs.27.8±1.7;32.9±1.5vs.29.8±1.7;32.0±3.8vs.分别为27.1±2.1）。基线mtDNA浓度在使用接受者操作特征分析预测严重创伤的IAI患者死亡方面具有很高的有效性。此外，入院时血清mtDNA水平与乳酸浓度相关，但是在mtDNA水平和白细胞水平之间没有发现显著的相关性，C反应蛋白,和降钙素原.
结论：血浆mtDNA与脓毒症的发生有关，MODS,严重腹部创伤导致的IAIs患者死亡。
方法：在医院第0天从IAIs患者的血清样本中分离DNA，一,还有两个.通过实时聚合酶链反应(PCR)评估血浆mtDNA浓度。分析研究人群的临床和实验室数据。
结果：使用四个选择的序列，将mtDNA损伤相关的分子模式表示为PCR阈值循环数。IAI患者的血浆mtDNA明显高于健康对照组。与具有IAIs的非脓毒症患者相比，具有IAIs的IAIs患者的mtDNA水平明显升高（30.9±2.0vs.28.7±2.4;33.3±2.6vs.28.9±2.4;32.9±1.6vs.31.2±2.2;33.1±3.6vs.分别为28.1±2.2)。与未发生多器官功能障碍综合征（MODS）的IAIs患者相比，其mtDNA浓度也增加了（31.0±1.8vs.27.9±1.8;32.9±2.4vs.27.8±1.7;32.9±1.5vs.29.8±1.7;32.0±3.8vs.分别为27.1±2.1）。基线mtDNA浓度在使用接受者操作特征分析预测严重创伤的IAI患者死亡方面具有很高的有效性。此外，入院时血清mtDNA水平与乳酸浓度相关，但是在mtDNA水平和白细胞水平之间没有发现显著的相关性，C反应蛋白,和降钙素原.
结论：血浆mtDNA与脓毒症的发生有关，MODS,严重腹部创伤导致的IAIs患者死亡。
