PDF(Sci-hub)
Abstract：
The cinnamon flounder Pseudorhombus cinnamoneus is a frequently consumed marine fish in China. However, the occurrence of ascaridoid larvae in P. cinnamoneus remains unclear. In the present study, a total of 85 P. cinnamoneus caught from the Yellow Sea (off Shidao, 36°52\'57″N, 122°26\'42″E) in 2011, which is located between mainland China and the Korean Peninsula, was investigated for ascaridoid larval infection. Four ascaridoid larval types, including Anisakis type I of Berland (1961), Hysterothylacium type of Smith (1983), Hysterothylacium type HL of Guo et al. (2014) and Raphidascaris type of Zhao et al. (2016), were detected in this important food fish. These larval types were identified as Anisakis pegreffii, Hysterothylacium aduncum, H. sinense and Raphidascaris lophii, respectively, using PCR-based restriction fragment length polymorphism (PCR-RFLP) analysis and sequencing of the ITS region of nuclear ribosomal DNA (rDNA). The third-stage larvae of H. sinense are reported from Chinese waters for the first time. The prevalence of H. sinense was 100% and represents the predominant species of the ascaridoid larvae found in P. cinnamoneus. The prevalences of A. pegreffii and H. aduncum were 44.7% and 81.2%, respectively. Phylogenetic analyses based on ITS sequences were performed to elucidate the genetic relationships of these ascaridoid nematodes. The present study increases the knowledge and distribution of ascaridoid larvae in this area of Yellow Sea. The high prevalence of ascaridoid larvae in P. cinnamoneus shows that an assessment needs to be undertaken to assess the risk these parasites may pose to public health.
摘要：
肉桂比目鱼是中国经常食用的海鱼。然而,目前尚不清楚在肉桂中是否存在类星虫幼虫。在本研究中,从黄海捕获的总共85片肉桂(石岛附近，36°52\'57″N，122°26\'42″E)，2011年，位于中国大陆和朝鲜半岛之间，被调查为asc虫幼虫感染。四种类虫幼虫类型，包括Berland(1961)的AnisakisI型，SterothylaciumtypesofSmith(1983),Guo等人的赤霉病HL型。(2014)和Zhao等人的Raphidascaris型。(2016),在这种重要的食用鱼中检测到。这些幼虫类型被鉴定为Anisakispegreffii，赤霉病，H.Sinense和Raphidascarislophii，分别,使用基于PCR的限制性片段长度多态性（PCR-RFLP）分析并测序细胞核核糖体DNA（rDNA）的ITS区域。首次从中国水域报道了H.sinense的第三阶段幼虫。H.sinense的患病率为100％，代表了在肉桂中发现的a虫幼虫的主要物种。A.pegreffii和H.aduncum的患病率分别为44.7％和81.2％，分别。进行了基于ITS序列的系统发育分析，以阐明这些A虫线虫的遗传关系。本研究增加了黄海该地区a虫幼虫的知识和分布。在肉桂中，类as虫幼虫的患病率很高，这表明需要进行评估以评估这些寄生虫可能对公众健康构成的风险。
