PDF(Pubmed)
Abstract：
Biodegradable zinc (Zn) metals, a new generation of biomaterials, have attracted much attention due to their excellent biodegradability, bioabsorbability, and adaptability to tissue regeneration. Compared with magnesium (Mg) and iron (Fe), Zn exhibits better corrosion and mechanical behaviors in orthopedic and stent applications. After implantation, Zn containing material will slowly degrade, and Zn ions (Zn2+) will be released to the surrounding tissue. For stent applications, the local Zn2+concentration near endothelial tissue/cells could be high. However, it is unclear how endothelia will respond to such high concentrations of Zn2+, which is pivotal to vascular remodeling and regeneration. Here, we evaluated the short-term cellular behaviors of primary human coronary artery endothelial cells (HCECs) exposed to a concentration gradient (0-140 μM) of extracellular Zn2+. Zn2+ had an interesting biphasic effect on cell viability, proliferation, spreading, and migration. Generally, low concentrations of Zn2+ promoted viability, proliferation, adhesion, and migration, while high concentrations of Zn2+ had opposite effects. For gene expression profiles, the most affected functional genes were related to cell adhesion, cell injury, cell growth, angiogenesis, inflammation, vessel tone, and coagulation. These results provide helpful information and guidance for Zn-based alloy design as well as the controlled release of Zn2+in stent and other related medical applications.
摘要：
可生物降解的锌（Zn）金属，新一代生物材料，由于其优异的生物降解性而备受关注，生物吸收性，以及对组织再生的适应性。与镁（Mg）和铁（Fe）相比，Zn在骨科和支架应用中表现出更好的腐蚀和机械性能。植入后,含锌材料会缓慢降解，和Zn离子(Zn2+)将被释放到周围组织。对于支架应用，内皮组织/细胞附近的局部Zn2+浓度可能较高。然而,目前尚不清楚内皮将如何响应如此高浓度的Zn2+，这对血管重塑和再生至关重要。这里,我们评估了暴露于细胞外Zn2浓度梯度（0-140μM）的原代人冠状动脉内皮细胞（HCECs）的短期细胞行为。Zn2+对细胞活力具有有趣的双相作用，扩散,传播,和移民。一般来说,低浓度的Zn2+促进了生存力，扩散,附着力,和移民,而高浓度的Zn2+有相反的作用。对于基因表达谱，受影响最大的功能基因与细胞粘附有关，细胞损伤,细胞生长,血管生成,炎症,血管音调，和凝结。这些结果为Zn基合金的设计以及Zn2+在支架和其他相关医学应用中的控释提供了有益的信息和指导。
