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Abstract：
The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 × 75 µm, frame rates of up to 3000 Hz and a dead time as low as 3.8 µs. An EIGER 1M and EIGER 16M were tested on Swiss Light Source beamlines X10SA and X06SA for their application in macromolecular crystallography. The combination of fast frame rates and a very short dead time allows high-quality data acquisition in a shorter time. The ultrafine ϕ-slicing data-collection method is introduced and validated and its application in finding the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose are presented. An improvement of the data quality up to slicing at one tenth of the mosaicity has been observed, which is much finer than expected based on previous findings. The influence of key data-collection parameters on data quality is discussed.
摘要：
单光子计数探测器的发展，比如PILATUS,是最近高分子晶体学的重大突破，实现无噪声检测和新颖的数据采集模式。新的EIGER探测器的像素尺寸为75×75µm，高达3000Hz的帧速率和低至3.8µs的死区时间。在瑞士光源光束线X10SA和X06SA上测试了EIGER1M和EIGER16M在大分子晶体学中的应用。快速帧速率和非常短的死区时间的组合允许在更短的时间内进行高质量的数据采集。介绍并验证了超精细切片数据采集方法及其在寻找最佳旋转角度中的应用，提出了合适的旋转速度和足够的X射线剂量。已经观察到数据质量的改善，直到以马赛克的十分之一进行切片，根据以前的发现，这比预期的要精细得多。讨论了关键数据收集参数对数据质量的影响。
