Toxicity of Bacillus thuringiensis crystal proteins against eri silkworm, Samia cynthia ricini (Lepidoptera: Saturniidae).

苏云金芽孢杆菌晶体蛋白对艾里蚕的毒性,菊科 （ 鳞翅目 ： 土星科 ） 。

• 影响指数: 2.795
• 发表时间: 07 2016
• 来源期刊: J Invertebr Pathol
• DOI: 10.1016/j.jip.2016.06.012
• PMID: 27377590
• 文章类型: Journal Article
• 中科院分区: Q1
• 作者列表: Sandeep Kumar D,Tarakeswari M,Lakshminarayana M,Sujatha M

关键词: Bacillus thuringiensis; Crystal proteins; Eri silkworm; Insect bioassays; Samia ricini

Mesh: Animals; Bacillus thuringiensis Toxins; Bacterial Proteins / toxicity; Bombyx; Endotoxins / toxicity; Hemolysin Proteins / toxicity; Insecticides; Pest Control, Biological / methods

Abstract

Ten purified crystal proteins of Bacillus thuringiensis (Bt) were tested at concentrations ranging from 2.93 to 3000ng/cm(2) for their toxicity to eri silkworm through protein paint bioassays using castor leaves. Based on LC50 values, Cry1Aa (2.6ng/cm(2)) was highly toxic followed by Cry1Ac (29.3ng/cm(2)) and Cry1Ab (68.7ng/cm(2)). The Cry1Ca and Cry1Ea proteins were moderately toxic to eri silkworm larvae and resulted in 23% and 28% mortality, respectively at the highest concentration tested (3000ng/cm(2)). Only reduction in larval weight was observed with Cry2Aa, Cry1Da and Cry9Aa proteins while Cry3Aa and Cry1Ba proteins were found to be nontoxic.

摘要

通过使用蓖麻叶的蛋白质涂料生物测定法，以2.93至3000ng/cm（2）的浓度测试了10种纯化的苏云金芽孢杆菌（Bt）晶体蛋白对埃里蚕的毒性。基于LC50值，Cry1Aa（2.6ng/cm（2））具有高毒性，其次是Cry1Ac（29.3ng/cm（2））和Cry1Ab（68.7ng/cm（2））。Cry1Ca和Cry1Ea蛋白对家蚕幼虫有中等毒性，导致23%和28%的死亡率，分别在最高浓度测试（3000ng/cm（2））。Cry2Aa只观察到幼虫重量的减少，发现Cry1Da和Cry9Aa蛋白而Cry3Aa和Cry1Ba蛋白是无毒的。