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Abstract：
The relationship among the lysyl oxidase (LOX) G473A single nucleotide polymorphism (SNP), cigarette smoking and lung, colorectal, colon and rectum cancer susceptibility was studied in 200 cases of lung cancer, 335 cases of colorectal cancer including 130 cases of colon cancer and 205 cases of rectum cancer, and 335 healthy people in Tangshan, China. Peripheral blood DNA samples were collected, DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) performed, followed by multivariate logistic regression analysis. In comparison to LOX473GG genotype carriers, individuals with LOX473AA exhibited a higher susceptibility to lung, colon-rectum, colon, and rectum cancers with OR values amounting to 3.84-, 2.74-, 2.75-, and 2.74-fold of the control, respectively. In the LOX 473AA-positive population, females were more susceptible than males to carcinogenesis with OR values (female vs. male): 5.25 vs. 3.23, 2.29 vs. 1.51, 2.27 vs. 1.45, and 2.25 vs. 1.53, respectively, for lung, colon-rectum combined, colon, and rectum cancers. LOX G473A polymorphism apparently elevated human sensitivity to cigarette smoking carcinogens for eliciting cancers in the lung and colon only. Thus, LOX G473A polymorphism positively correlates with carcinogenesis and it may be used as an ideal intrinsic biomarker for prediction or diagnosis of carcinogenesis in humans.
摘要：
赖氨酰氧化酶(LOX)G473A单核苷酸多态性(SNP),吸烟和肺，结直肠,在200例肺癌中研究了结肠癌和直肠癌的易感性，结直肠癌335例,其中结肠癌130例,直肠癌205例,唐山335名健康人，中国。收集外周血DNA样本,进行DNA测序和聚合酶链反应-限制性片段长度多态性（PCR-RFLP），其次是多因素logistic回归分析。与LOX473GG基因型携带者相比，LOX473AA患者对肺的易感性更高，结肠直肠,结肠,和OR值为3.84-的直肠癌，2.74-,2.75-，和2.74倍的对照，分别。在LOX473AA阳性人群中，女性比男性更容易发生癌症，其OR值(女性与男性）：5.25vs.3.23,2.29vs.1.51,2.27vs.1.45和2.25vs.1.53，分别对于肺，结肠-直肠联合，结肠,和直肠癌.LOXG473A多态性明显提高了人类对吸烟致癌物的敏感性，仅在肺癌和结肠癌中引起癌症。因此，LOXG473A多态性与癌变呈正相关，可作为预测或诊断人类癌变的理想内在生物标志物。
