P(VDF-TrFE)/BaTiO3 Nanoparticle Composite Films Mediate Piezoelectric Stimulation and Promote Differentiation of SH-SY5Y Neuroblastoma Cells.

• 影响指数: 11.092
• 发表时间: 07 2016
• 来源期刊: Adv Healthc Mater
• DOI: 10.1002/adhm.201600245
• PMID: 27283784
• 文章类型: Journal Article
• 中科院分区: Q1
• 作者列表: Genchi GG,Ceseracciu L,Marino A,Labardi M,Marras S,Pignatelli F,Bruschini L,Mattoli V,Ciofani G

关键词: BaTiO3 nanoparticles; P(VDF-TrFE); neurons; piezoelectric stimulation

Mesh: Barium Compounds / chemistry; Cell Differentiation; Cell Line, Tumor; Cell Survival; Electric Stimulation / methods; Humans; Polyvinyls / chemistry; Titanium / chemistry

Abstract

Poly(vinylidene fluoride-trifluoroethylene, P(VDF-TrFE)) and P(VDF-TrFE)/barium titanate nanoparticle (BTNP) films are prepared and tested as substrates for neuronal stimulation through direct piezoelectric effect. Films are characterized in terms of surface, mechanical, and piezoelectric features before in vitro testing on SH-SY5Y cells. In particular, BTNPs significantly improve piezoelectric properties of the films (4.5-fold increased d31 ). Both kinds of films support good SH-SY5Y viability and differentiation. Ultrasound (US) stimulation is proven to elicit Ca(2+) transients and to enhance differentiation in cells grown on the piezoelectric substrates. For the first time in the literature, this study demonstrates the suitability of polymer/ceramic composite films and US for neuronal stimulation through direct piezoelectric effect.