PDF(Pubmed)
Abstract：
Embryonic stem cells have unlimited proliferative capacity, which may provide a source of tendon stem/progenitor cells for tissue engineering. Experts of International Science and Technology Collaborative Program of Ministry of Science and Technology have developed a protocol consensus on differentiation of human embryonic stem cells into the tendon cells. The consensus recommends a protocol of two-step generation of human embryonic stem cells into tendon cells: the human embryonic stem cells are first differentiated into mesenchymal stem cells on different material surfaces; then with the scaffold-free tissue engineering tendon formed by high-density planting, the mesenchymal stem cells are induced into tendon cells under static or dynamic mechanical stimulation in vivo and in vitro. Tissue engineering tendon established in vitro by the protocol can be used as a model in toxicological analysis and safety evaluation of tendon-relevant small molecule compounds, medical materials and drugs.
摘要：
胚胎干细胞具有无限的增殖能力，这可能为组织工程提供肌腱干/祖细胞的来源。科学技术部国际科学技术合作计划的专家已经就人类胚胎干细胞分化为肌腱细胞达成了协议共识。共识推荐了人胚胎干细胞两步生成肌腱细胞的方案：人胚胎干细胞首先在不同的材料表面分化成间充质干细胞；然后通过高密度种植形成的无支架组织工程肌腱，在体内和体外静态或动态机械刺激下，间充质干细胞被诱导为肌腱细胞。通过该方案在体外建立的组织工程肌腱可作为肌腱相关小分子化合物的毒理学分析和安全性评价模型。医疗材料和药品。
