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Abstract：
A new method for determining nucleotide sequences in DNA is described. It is similar to the \"plus and minus\" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2\',3\'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.
摘要：
描述了一种确定DNA中核苷酸序列的新方法。它类似于“加减”方法[桑格，F.&Coulson,A.R.(1975)J.Mol。Biol.94，441-448]，但利用了2\'，正常脱氧核苷三磷酸的3'-双脱氧和阿拉伯核苷类似物，作为DNA聚合酶的特异性链终止抑制剂。该技术已应用于噬菌体varphiX174的DNA，比加号或减号方法更快，更准确。
