Abstract：
The transcription factor GATA-1 plays an essential role in erythroid differentiation. To identify novel GATA-1 target genes, we analyzed a merged ChIP-seq and expression profiling dataset. We identified FAM210B as a putative novel GATA-1 target gene. Study results demonstrated that GATA-1 directly regulates FAM210B expression, presumably by binding to an intronic enhancer region. Both human and murine FAM210B are abundantly expressed in the later stages of erythroblast development. Moreover, the deduced amino acid sequence predicted that FAM210B is a membrane protein, and Western blot analysis demonstrated its mitochondrial localization. Loss-of-function analysis in erythroid cells suggested that FAM210B may be involved in erythroid differentiation. The identification and characterization of FAM210B provides new insights in the study of erythropoiesis and hereditary anemias.
摘要：
转录因子GATA-1在类红细胞分化中起重要作用。为了鉴定新的GATA-1靶基因，我们分析了合并的ChIP-seq和表达谱数据集。我们将FAM210B鉴定为推定的新型GATA-1靶基因。研究结果表明GATA-1直接调控FAM210B的表达,大概是通过与内含子增强子区域结合。人和鼠FAM210B都在成红细胞发育的后期阶段大量表达。此外，推导的氨基酸序列预测FAM210B是一种膜蛋白，和Western印迹分析证明了其线粒体定位。红系细胞功能缺失分析提示FAM210B可能参与红系分化。FAM210B的鉴定和表征为红细胞生成和遗传性贫血的研究提供了新的见解。
