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Abstract：
In a study where the prevalence of Aeromonas in shellfish was analysed, three isolates of Aeromonas schubertii were identified, representing this the first report of this species from mussels. This species was originally described in 1988 from strains isolated from extra-intestinal human infections and since then has been cited in only 18 occasions. For many years, A. schubertii was the only mannitol-negative species of the genus. However, three additional mannitol-negative species (Aeromonas simiae, Aeromonas diversa and Aeromonas australiensis) have been described. This, together with the fact that A. schubertii is a rare human pathogenic species, motivated the present study to characterize its biochemical behaviour and differentiation from the other mannitol-negative species. The molecular similarity (16S rRNA, rpoD and gyrB genes) of the strains, presence of virulence genes and antimicrobial resistance were determined. All A. schubertii strains showed the same phenotypic behaviour, i.e. they use citrate, are positive for lysine decarboxylase and DL-lactate, but negative for production of mannitol, indole and acid from sucrose and could be easily differentiated from other mannitol-negative species. All strains carried the aerA and lafA virulence genes and showed susceptibility to all antibiotics tested. Seafood could be a transmission route of this bacterium to humans.
摘要：
在一项分析贝类中气单胞菌流行率的研究中，鉴定出了三种舒伯氏气单胞菌，代表这是贻贝对该物种的首次报道。该物种最初是在1988年从肠道外人类感染中分离出的菌株中描述的，此后仅被引用了18次。多年来,A.schubertii是该属中唯一的甘露醇阴性物种。然而,另外三个甘露醇阴性物种(Aeromonassimiae，已经描述了双气单胞菌和澳大利亚气单胞菌）。这个,加上A.schubertii是一种罕见的人类致病物种，促使本研究表征其生化行为和与其他甘露醇阴性物种的区别。分子相似性(16SrRNA，菌株的rpoD和gyrB基因），确定了毒力基因的存在和抗菌素耐药性。所有的舒伯蒂A.schubertii菌株都表现出相同的表型行为，即他们使用柠檬酸盐，赖氨酸脱羧酶和DL-乳酸呈阳性，但对甘露醇的生产不利，吲哚和蔗糖酸，可以很容易地与其他甘露醇阴性物种区分开。所有菌株均携带aerA和lafA毒力基因，并对所有测试的抗生素均具有敏感性。海鲜可能是这种细菌向人类传播的途径。
