PDF(Pubmed)
Abstract：
OBJECTIVE: In traditional medicine Salvia officinalis (sage) has been used as menstrual cycle regulator. In the present study the effects of sage extract on breast tissue were examined.
METHODS: Fourteen female rats were divided into two groups: 1) Distilled water-treated rats (Con) that were gavaged with 1ml distilled water and 2) Saliva officinalis hydroalcoholic extract (SHE)-treated rats that were gavaged with 30mg/kg/body weight of sage extract for 30 days. The estrus cycle changes were monitored by daily examination of vaginal smear. Whole mounts of right pelvic breast were spread on the slide and stained by carmine. The number of alveolar buds (ABs) type 1 and 2 and lobules of mammary gland were scored. Tissue sections of left pelvic mammary gland were prepared and its histomorphometrical changes were measured. Blood samples were taken from dorsal aorta and estradiol and progesterone concentrations were measured using radioimmunoassay.
RESULTS: Estrous cycles decreased significantly in SHE-treated animals. The number of alveolar buds and lobules in mammary gland whole mount of SHE-treated group were higher than the Con group. The number and diameter of ducts in histological section of mammary gland in SHE-treated group increased as compared to the Con group.
CONCLUSIONS: Sage promotes alveologenesis of mammary glands and it can be used as a lactiferous herb.
METHODS: Fourteen female rats were divided into two groups: 1) Distilled water-treated rats (Con) that were gavaged with 1ml distilled water and 2) Saliva officinalis hydroalcoholic extract (SHE)-treated rats that were gavaged with 30mg/kg/body weight of sage extract for 30 days. The estrus cycle changes were monitored by daily examination of vaginal smear. Whole mounts of right pelvic breast were spread on the slide and stained by carmine. The number of alveolar buds (ABs) type 1 and 2 and lobules of mammary gland were scored. Tissue sections of left pelvic mammary gland were prepared and its histomorphometrical changes were measured. Blood samples were taken from dorsal aorta and estradiol and progesterone concentrations were measured using radioimmunoassay.
RESULTS: Estrous cycles decreased significantly in SHE-treated animals. The number of alveolar buds and lobules in mammary gland whole mount of SHE-treated group were higher than the Con group. The number and diameter of ducts in histological section of mammary gland in SHE-treated group increased as compared to the Con group.
CONCLUSIONS: Sage promotes alveologenesis of mammary glands and it can be used as a lactiferous herb.
摘要：
目的：在传统医学中，丹参（鼠尾草）已被用作月经周期调节剂。在本研究中，研究了鼠尾草提取物对乳腺组织的影响。
方法：将14只雌性大鼠分为两组：1）用1ml蒸馏水灌胃的蒸馏水处理的大鼠（Con）和2）用30mg/kg/体重的鼠尾草提取物（SHE）处理的大鼠灌胃30天。通过每日检查阴道涂片来监测发情周期的变化。将整个右侧骨盆乳房铺展在载玻片上，并被胭脂红染色。对乳腺1型和2型肺泡芽（ABs）和小叶的数量进行评分。制备左盆腔乳腺组织切片并测量其组织形态变化。从背主动脉采集血样，并使用放射免疫分析法测量雌二醇和孕酮的浓度。
结果:在SHE处理的动物中,发情周期显著降低。SHE治疗组乳腺整体的肺泡芽和小叶数量高于Con组。与Con组相比，SHE治疗组乳腺组织学切片中导管的数量和直径增加。
结论：鼠尾草可促进乳腺的肺泡形成，可用作乳草。
方法：将14只雌性大鼠分为两组：1）用1ml蒸馏水灌胃的蒸馏水处理的大鼠（Con）和2）用30mg/kg/体重的鼠尾草提取物（SHE）处理的大鼠灌胃30天。通过每日检查阴道涂片来监测发情周期的变化。将整个右侧骨盆乳房铺展在载玻片上，并被胭脂红染色。对乳腺1型和2型肺泡芽（ABs）和小叶的数量进行评分。制备左盆腔乳腺组织切片并测量其组织形态变化。从背主动脉采集血样，并使用放射免疫分析法测量雌二醇和孕酮的浓度。
结果:在SHE处理的动物中,发情周期显著降低。SHE治疗组乳腺整体的肺泡芽和小叶数量高于Con组。与Con组相比，SHE治疗组乳腺组织学切片中导管的数量和直径增加。
结论：鼠尾草可促进乳腺的肺泡形成，可用作乳草。
