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Abstract：
BACKGROUND: Paediatric respiratory tract infections are among the most common reasons for preschool and school absences and visits to physicians. The disease mainly involves the upper respiratory tract and is associated with fever, cough, sore throat, and running nose. Children with recurrent respiratory infections (RRI), which are defined as more than six serious diseases a year, are a difficult diagnostic challenge. The aim of this study was to assess immunological deviations in laboratory tests performed in children with RRI.
METHODS: In the retrospective study 25 children suffering from recurrent respiratory tract infection, aged 4.1 ±2.3 years, 13 boys and 12 girls, were involved. For all children chemiluminescence of granulocytes and immunophenotyping of lymphocytes from peripheral blood were examined. An immunophenotype of peripheral blood lymphocytes involved evaluation of T cell, B cells, and NK cells, examined with flow cytometry.
RESULTS: Eleven of the studied children had decreased chemiluminescent response to stimulants, normal response was found for nine children, and five children had an increased result of the test. Five of the 25 children had decreased B cells number, and five had decreased number of T cells including decrease of CD4, as well as CD8 positive cells. Children with decreased chemiluminescence had more frequent neutropaenia than children with normal or increased chemiluminescent response, p < 0.05 (exact Fisher test).
CONCLUSIONS: Recurrent respiratory tract infection could be associated with improper neutrophils response to pathogens, and immunological examination should be performed to find the reason for the increased number of infections in a year.
METHODS: In the retrospective study 25 children suffering from recurrent respiratory tract infection, aged 4.1 ±2.3 years, 13 boys and 12 girls, were involved. For all children chemiluminescence of granulocytes and immunophenotyping of lymphocytes from peripheral blood were examined. An immunophenotype of peripheral blood lymphocytes involved evaluation of T cell, B cells, and NK cells, examined with flow cytometry.
RESULTS: Eleven of the studied children had decreased chemiluminescent response to stimulants, normal response was found for nine children, and five children had an increased result of the test. Five of the 25 children had decreased B cells number, and five had decreased number of T cells including decrease of CD4, as well as CD8 positive cells. Children with decreased chemiluminescence had more frequent neutropaenia than children with normal or increased chemiluminescent response, p < 0.05 (exact Fisher test).
CONCLUSIONS: Recurrent respiratory tract infection could be associated with improper neutrophils response to pathogens, and immunological examination should be performed to find the reason for the increased number of infections in a year.
摘要：
背景：儿科呼吸道感染是学龄前和学校缺勤以及去看医生的最常见原因之一。该病主要累及上呼吸道,并伴有发热,咳嗽,喉咙痛,流鼻涕。反复呼吸道感染（RRI）的儿童，被定义为每年超过六种严重疾病，是一个艰难的诊断挑战。这项研究的目的是评估RRI儿童实验室检查中的免疫学偏差。
方法：在回顾性研究中，25名反复呼吸道感染的儿童，年龄为4.1±2.3岁，13个男孩和12个女孩，参与其中。对于所有儿童，都检查了粒细胞的化学发光和外周血淋巴细胞的免疫表型。外周血淋巴细胞的免疫表型涉及T细胞的评估，B细胞,和NK细胞，用流式细胞仪检查。
结果：研究儿童中有11人对兴奋剂的化学发光反应降低，九个孩子的反应正常，五个孩子的测试结果有所提高。25名儿童中有5名B细胞数量减少，5例T细胞数量减少，包括CD4和CD8阳性细胞减少。化学发光减少的儿童比化学发光反应正常或增加的儿童更频繁的中性粒细胞减少症。p<0.05(精确Fisher检验)。
结论：反复呼吸道感染可能与中性粒细胞对病原体的反应不当有关，应进行免疫检查，以找出一年内感染人数增加的原因。
方法：在回顾性研究中，25名反复呼吸道感染的儿童，年龄为4.1±2.3岁，13个男孩和12个女孩，参与其中。对于所有儿童，都检查了粒细胞的化学发光和外周血淋巴细胞的免疫表型。外周血淋巴细胞的免疫表型涉及T细胞的评估，B细胞,和NK细胞，用流式细胞仪检查。
结果：研究儿童中有11人对兴奋剂的化学发光反应降低，九个孩子的反应正常，五个孩子的测试结果有所提高。25名儿童中有5名B细胞数量减少，5例T细胞数量减少，包括CD4和CD8阳性细胞减少。化学发光减少的儿童比化学发光反应正常或增加的儿童更频繁的中性粒细胞减少症。p<0.05(精确Fisher检验)。
结论：反复呼吸道感染可能与中性粒细胞对病原体的反应不当有关，应进行免疫检查，以找出一年内感染人数增加的原因。
