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Abstract：
In this paper, we report a high-throughput biological method to prepare spore-based monodisperse microparticles (SMMs) and then form the nanocomposites of CdTe quantum dot (QD)-loaded SMMs by utilizing the endogenous functional groups from Bacillus spores. The SMMs and QD-incorporated spore microspheres (QDSMs) were characterized by using transmission electron microscopy, high-resolution transmission electron microscopy, fluorescence microscopy, fluorescence and UV-visible absorption spectroscopy, zeta potential analysis, Fourier-transform infrared spectroscopy, potentiometric titrations, X-ray photo-electron spectroscopy. The thermodynamics of QD/SMM interaction and antigen/QDSM interaction was also investigated by isothermal titration microcalorimetry (ITC). Fluorescent QDSMs coded either with a single luminescence color or with multiple colors of controlled emission intensity ratios were obtained. Green QDSMs were used as a model system to detect porcine parvovirus antibody in swine sera via flow cytometry, and the results demonstrated a great potential of QDSMs in high-throughput immunoassays. Due to the advantages such as simplicity, low cost, high throughput and eco-friendliness, our developed platform may find wide applications in disease detection, food safety evaluation and environmental assessment.
摘要：
在本文中,我们报道了一种高通量的生物方法来制备基于孢子的单分散微粒（SMMs），然后通过利用芽孢杆菌孢子的内源性官能团形成载有CdTe量子点（QD）的SMMs的纳米复合材料。使用透射电子显微镜对SMM和QD掺入的孢子微球（QDSM）进行了表征，高分辨率透射电子显微镜，荧光显微镜,荧光和紫外可见吸收光谱，zeta电位分析，傅里叶变换红外光谱,电位滴定,X射线光电子能谱。还通过等温滴定微量热法（ITC）研究了QD/SMM相互作用和抗原/QDSM相互作用的热力学。获得了以单一发光颜色或具有受控发射强度比的多种颜色编码的荧光QDSM。以绿色QDSMs为模型系统，通过流式细胞术检测猪血清中的猪细小病毒抗体，结果证明了QDSM在高通量免疫分析中的巨大潜力。由于简单等优点，低成本,高通量和生态友好，我们开发的平台可以在疾病检测中找到广泛的应用，食品安全评价和环境评价。
