关键词: Molecular epidemiology Salmonella typing outbreaks public health microbiology surveillance

Mesh : Adult Aged Aged, 80 and over Disease Outbreaks Electrophoresis, Gel, Pulsed-Field Female Genome Humans Ireland / epidemiology Male Middle Aged Minisatellite Repeats Multilocus Sequence Typing Phylogeny Salmonella Infections / epidemiology microbiology Salmonella enterica / classification genetics Sequence Analysis, DNA

来  源:   DOI:10.1017/S0950268815001636   PDF(Sci-hub)

Abstract:
Salmonella enterica subsp. enterica serovar Dublin is an uncommon cause of human salmonellosis; however, a relatively high proportion of cases are associated with invasive disease. The serotype is associated with cattle. A geographically diffuse outbreak of S. Dublin involving nine patients occurred in Ireland in 2013. The source of infection was not identified. Typing of outbreak associated isolates by pulsed-field gel electrophoresis (PFGE) was of limited value because PFGE has limited discriminatory power for S. Dublin. Whole genome sequencing (WGS) showed conclusively that the isolates were closely related to each other, to an apparently unrelated isolate from 2011 and distinct from other isolates that were not readily distinguishable by PFGE.
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