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Abstract：
Quantitative phase microscopy (QPM) has recently emerged as a new powerful quantitative imaging technique well suited to noninvasively explore a transparent specimen with a nanometric axial sensitivity. In this review, we expose the recent developments of quantitative phase-digital holographic microscopy (QP-DHM). Quantitative phase-digital holographic microscopy (QP-DHM) represents an important and efficient quantitative phase method to explore cell structure and dynamics. In a second part, the most relevant QPM applications in the field of cell biology are summarized. A particular emphasis is placed on the original biological information, which can be derived from the quantitative phase signal. In a third part, recent applications obtained, with QP-DHM in the field of cellular neuroscience, namely the possibility to optically resolve neuronal network activity and spine dynamics, are presented. Furthermore, potential applications of QPM related to psychiatry through the identification of new and original cell biomarkers that, when combined with a range of other biomarkers, could significantly contribute to the determination of high risk developmental trajectories for psychiatric disorders, are discussed.
摘要：
定量相显微镜（QPM）最近已成为一种新的强大的定量成像技术，非常适合非侵入性地探索具有纳米轴向灵敏度的透明样品。在这次审查中,我们揭示了定量相数字全息显微镜（QP-DHM）的最新进展。定量相位数字全息显微镜（QP-DHM）是一种重要而有效的定量相位方法，用于探索细胞结构和动力学。在第二部分,总结了QPM在细胞生物学领域中最相关的应用。特别强调原始生物信息，可以从定量相位信号中得出。在第三部分,最近获得的应用，QP-DHM在细胞神经科学领域，即光学解析神经元网络活动和脊柱动力学的可能性，被呈现。此外，通过鉴定新的和原始的细胞生物标志物，QPM与精神病学相关的潜在应用，当与一系列其他生物标志物结合时，可以显着有助于确定精神疾病的高风险发育轨迹，正在讨论。
