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Abstract：
OBJECTIVE: In this study, we aimed to measure changes in red blood cell (RBC) immunity and cytokine levels after performing total hip replacement surgery.
METHODS: Twenty patients receiving total hip arthroplasty were investigated by measuring presurgical and postoperative RBC natural tumor erythrocyte rosette rate (NTERR), RBC C3b receptor rosette rate (RC3bRR), RBC membrane CD35, CD58 and CD59 expression and cytokine levels [including tumor necrosis factor α (TNF-α), interleukin 2 (IL-2), interferon γ (IFN-γ), interleukin 10 (IL-10) and prostaglandin E2 (PGE2)]. Blood samples were collected on the day before surgery and on the first day after hip arthroplasty.
RESULTS: Postoperative NTERR and RC3bRR were significantly lower than presurgical levels (p < 0.05). The RBC membrane CD35, CD58 and CD59 expressions were significantly decreased in the postoperative phase compared to pre-operative levels. Importantly, RBC promoting lymphocyte proliferation rates were significantly reduced after surgery. In addition, postoperative TNF-α, IL-2 and IFN-γ levels in RBC and lymphocyte culture fluid were lower than those pre-operation, whereas IL-10 and PGE2 were significantly increased compared to presurgical levels (p < 0.05).
CONCLUSIONS: The modification of RBC immune function may be involved in the occurrence and development of the infection following hip arthroplasty, and this suggests a novel strategy to prevent such infection.
METHODS: Twenty patients receiving total hip arthroplasty were investigated by measuring presurgical and postoperative RBC natural tumor erythrocyte rosette rate (NTERR), RBC C3b receptor rosette rate (RC3bRR), RBC membrane CD35, CD58 and CD59 expression and cytokine levels [including tumor necrosis factor α (TNF-α), interleukin 2 (IL-2), interferon γ (IFN-γ), interleukin 10 (IL-10) and prostaglandin E2 (PGE2)]. Blood samples were collected on the day before surgery and on the first day after hip arthroplasty.
RESULTS: Postoperative NTERR and RC3bRR were significantly lower than presurgical levels (p < 0.05). The RBC membrane CD35, CD58 and CD59 expressions were significantly decreased in the postoperative phase compared to pre-operative levels. Importantly, RBC promoting lymphocyte proliferation rates were significantly reduced after surgery. In addition, postoperative TNF-α, IL-2 and IFN-γ levels in RBC and lymphocyte culture fluid were lower than those pre-operation, whereas IL-10 and PGE2 were significantly increased compared to presurgical levels (p < 0.05).
CONCLUSIONS: The modification of RBC immune function may be involved in the occurrence and development of the infection following hip arthroplasty, and this suggests a novel strategy to prevent such infection.
摘要：
目的：在本研究中，我们旨在测量全髋关节置换手术后红细胞(RBC)免疫和细胞因子水平的变化.
方法：通过测量术前和术后RBC自然肿瘤红细胞花环率（NTERR），对20例接受全髋关节置换术的患者进行了调查。红细胞C3b受体花环率（RC3bRR），红细胞膜CD35、CD58和CD59表达及细胞因子水平[包括肿瘤坏死因子α(TNF-α),白细胞介素2（IL-2），干扰素γ（IFN-γ），白细胞介素10（IL-10）和前列腺素E2（PGE2）]。在手术前一天和髋关节置换术后的第一天收集血液样品。
结果：术后NTERR和RC3bRR明显低于术前水平（p<0.05）。术后RBC膜CD35、CD58和CD59的表达较术前水平明显降低。重要的是,RBC促淋巴细胞增殖率术后显著降低。此外,术后TNF-α,红细胞和淋巴细胞培养液中IL-2和IFN-γ水平低于术前,而IL-10和PGE2与术前水平相比显着增加（p<0.05）。
结论：红细胞免疫功能的改变可能参与髋关节置换术后感染的发生和发展。这表明了一种预防这种感染的新策略。
方法：通过测量术前和术后RBC自然肿瘤红细胞花环率（NTERR），对20例接受全髋关节置换术的患者进行了调查。红细胞C3b受体花环率（RC3bRR），红细胞膜CD35、CD58和CD59表达及细胞因子水平[包括肿瘤坏死因子α(TNF-α),白细胞介素2（IL-2），干扰素γ（IFN-γ），白细胞介素10（IL-10）和前列腺素E2（PGE2）]。在手术前一天和髋关节置换术后的第一天收集血液样品。
结果：术后NTERR和RC3bRR明显低于术前水平（p<0.05）。术后RBC膜CD35、CD58和CD59的表达较术前水平明显降低。重要的是,RBC促淋巴细胞增殖率术后显著降低。此外,术后TNF-α,红细胞和淋巴细胞培养液中IL-2和IFN-γ水平低于术前,而IL-10和PGE2与术前水平相比显着增加（p<0.05）。
结论：红细胞免疫功能的改变可能参与髋关节置换术后感染的发生和发展。这表明了一种预防这种感染的新策略。
