RESULTS: Primary chondrocytes were isolated from Hoxc8- and Hoxd4-transgenic mouse embryo rib cartilage at 18.5 days of gestation. In both cases, differentially expressed genes were identified that have a role in cell proliferation and cell cycle regulation. A comparison between the controls for both experimental groups did not reveal significant differences, as expected. However, the repertoires of differentially expressed genes were found not to overlap between Hoxc8- and Hoxd4-transgenic cartilage. This included different Wnt genes, cell cycle, and apoptosis regulators.
CONCLUSIONS: Overexpression of Hoxc8 and Hoxd4 transcription factors alters transcriptional profiles in chondrocytes at E18.5. The differences in repertoires of altered gene expression between the 2 transgenic conditions suggest that the molecular mechanisms underlying the cartilage defects may be different in both transgenic paradigms, despite apparently similar phenotypes.
结果:在妊娠18.5天从Hoxc8和Hoxd4转基因小鼠胚胎肋软骨中分离出原代软骨细胞。在这两种情况下,差异表达的基因被鉴定为在细胞增殖和细胞周期调节中起作用。两个实验组的对照之间的比较没有发现显着差异,如预期。然而,差异表达基因库在Hoxc8-和Hoxd4-转基因软骨之间没有重叠。这包括不同的Wnt基因,细胞周期,和凋亡调节剂。
结论:在E18.5时,Hoxc8和Hoxd4转录因子的过表达改变了软骨细胞的转录谱。两种转基因条件之间改变的基因表达库的差异表明,两种转基因范式中软骨缺损的分子机制可能不同。尽管表型相似。