Correlation between B7-H3 expression and rheumatoid arthritis: A new polymorphism haplotype is associated with increased disease risk.

• 影响指数: 10.19
• 发表时间: Jul 2015
• 来源期刊: Clin Immunol
• DOI: 10.1016/j.clim.2015.04.012
• PMID: 25931383
• 文章类型: Journal Article
• 中科院分区: Q2
• 方向: 医学
• 作者列表: Sun J,Liu C,Gao L,Guo Y,Zhang Y,Wu P,Jiang J,Yan R,Zhang X

关键词: B7-H3/CD276; Gene expression; Rheumatoid arthritis (RA); Single nucleotide polymorphisms (SNPs)

Mesh: Arthritis, Rheumatoid / genetics metabolism; B7 Antigens / genetics metabolism pharmacology; Case-Control Studies; Cell Line; Genetic Predisposition to Disease; Haplotypes; Humans; Macrophages / drug effects metabolism; Monocytes / metabolism; Polymorphism, Single Nucleotide; RNA, Messenger / metabolism; Real-Time Polymerase Chain Reaction; Severity of Illness Index; Tumor Necrosis Factor-alpha / drug effects metabolism; Up-Regulation

Abstract

CD276 (B7-H3) is a costimulatory molecule that plays a potent role in T cell responses, however, the role of B7-H3 in autoimmune diseases has not been elucidated. We analyzed B7-H3 expression in rheumatoid arthritis (RA) for the first time and found B7-H3 was significantly up-regulated on monocytes in RA patients, while the levels of soluble B7-H3 in serum were lower than in controls (P < 0.0001). These differences correlated with clinical and laboratory disease parameters and informatory factor TNF-α. Through in vitro experiments, we demonstrated that B7-H3 promoted TNF-α secretion. In addition, a new polymorphism variant, B7-H3-T-A-C-T, was identified and shown to be associated with the incidence of RA and the decreased release of sB7-H3. These results suggest that B7-H3 may be a promising biomarker associated with the pathogenesis of RA. Notably, the new B7-H3-T-A-C-T polymorphism variant is associated with RA risk and might be associated with the release of soluble B7-H3.