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Abstract：
The fructans, inulin and oligofructose, were known to possess many physiologic properties. In the present study, the Box-Behnken design was used to determine the optimum extraction conditions of fructan from Eremurus spectabilis root powder (Serish) with water extraction, direct and indirect ultrasound assisted extraction methods that gave the maximum yield. Sonication amplitude (20-100 %), sonication temperature (30-70 °C) and sonication time (5-40 min) were considered variables of direct and indirect ultrasound extractions while for conventional extraction the following variables were water to solid ratio (30-50 v/w), temperature (40-90 °C) and time (5-40 min). A second-order polynomial model was fitted to each response and the regression coefficients were determined using least square method. There was a good agreement between the experimental data and their predicted counterparts. In addition to establishing the difference of these extraction methods, the scanning electron microscopy, Fourier-transform infrared spectroscopy, zeta potential and particle size analysis have been shown to be useful tools to investigate, approximate and predict characteristics of extracted fructan. Moreover, comparison of conventional extraction, direct sonication extraction, indirect sonication extraction showed the indirect sonication extraction is a suitable method for fructan extraction.
摘要：
果聚糖,菊粉和低聚果糖，已知具有许多生理特性。在本研究中,采用Box-Behnken设计确定了水提法从小木耳根粉（Serish）中提取果聚糖的最佳条件，直接和间接超声辅助提取方法可获得最大产量。超声振幅(20-100%),超声处理温度（30-70°C）和超声处理时间（5-40分钟）被认为是直接和间接超声提取的变量，而对于常规提取，以下变量是水固比（30-50v/w），温度(40-90°C)和时间(5-40分钟)。将二阶多项式模型拟合到每个响应，并使用最小二乘法确定回归系数。实验数据与预测数据之间有很好的一致性。除了建立这些提取方法的差异外，扫描电子显微镜，傅里叶变换红外光谱,zeta电位和粒度分析已被证明是有用的工具，以调查，提取果聚糖的近似和预测特征。此外，常规提取的比较，直接超声提取，间接超声提取表明，间接超声提取是果聚糖提取的合适方法。
