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Abstract：
Eccrine sweat glands are comprised of secretory coils and ducts, which are distinct in morphology and function. To better understand the roles of the two parts in development, homeostasis, wound repair and regeneration of eccrine sweat glands, we must distinguish between them. In this study, the localization of keratins and alpha-SMA in human eccrine sweat glands was examined by immunofluorescence staining. Based on the differential localization of keratins and alpha-SMA in different cell types, four pairs of antibodies (K5/K7, K5/alpha-SMA, K14/K7 and K14/alpha-SMA) were used to differentiate secretory coils from ducts by double-immunofluorescence staining. Immunofluorescence staining showed that myoepithelial cells of secretory coils expressed K5, K14 and alpha-SMA, whereas secretory cells of secretory coils expressed K7, K8, K15, K18 and K19. Ductal cells expressed K5, K8, K14 and K19. Double-staining showed that the secretory coils were K5(+)/K7(+), K5(+)/alpha-SMA(+), K14(+)/K7(+) and K14(+)/alpha-SMA(+), whereas ducts were K5(+)/K7(-), K5(+)/alpha-SMA(-), K14(+)/K7(-) and K14(+)/alpha-SMA(-). In conclusion, by combining use of keratins and alpha-SMA antibodies, secretory coils can be easily differentiated from ducts in morphology.
摘要：
汗腺由分泌线圈和导管组成，在形态和功能上截然不同。为了更好地理解这两个部分在开发中的作用，稳态，伤口修复和再生内分泌汗腺，我们必须区分它们。在这项研究中,通过免疫荧光染色检查人内分泌汗腺中角蛋白和α-SMA的定位。基于角蛋白和α-SMA在不同细胞类型中的差异定位,四对抗体（K5/K7，K5/α-SMA，K14/K7和K14/α-SMA）用于通过双免疫荧光染色区分导管中的分泌线圈。免疫荧光染色显示分泌线圈肌上皮细胞表达K5、K14和α-SMA,而分泌线圈的分泌细胞表达K7，K8，K15，K18和K19。导管细胞表达K5、K8、K14和K19。双重染色显示分泌线圈为K5(+)/K7(+),K5(+)/alpha-SMA(+),K14(+)/K7(+)和K14(+)/α-SMA(+)，而导管为K5(+)/K7(-)，K5(+)/α-SMA(-),K14(+)/K7(-)和K14(+)/α-SMA(-)。总之,通过结合使用角蛋白和α-SMA抗体，分泌线圈在形态上很容易与导管区分开。
