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Abstract：
Spore-forming bacterial strains were isolated from chicken gastrointestinal tracts to develop a heat-stable feed supplement that promotes weight gain in broilers. Seven Bacillus strains having more than 90% sporulation were screened from the isolates and identified to be closely related with Bacillus subtilis and Bacillus licheniformis. Of the seven strains, B. subtilis CH16 was selected to develop a feed supplement for broilers, because it formed 100% heat-stable spores, grew rapidly at 42°C and quickly formed a biofilm. In large-scale trials in broilers (n ≥ 1150 per group), the group fed CH16 (3 × 10(6) CFU g(-1) pellet) showed higher average daily gain (ADG = 61·16) and lower food conversion ratio (FCR = 1·696) than did the group fed B. licheniformis CH22 (ADG = 57·10 and FCR = 1·792), the group fed B. subtilis HU58 (ADG = 51·90 and FCR = 1·868), BioPlus group (ADG = 59·32 and FCR = 1·807) and the control group (ADG = 56·02 and FCR = 1·880). In conclusion, CH16 spores significantly increased ADG by 9·17% and reduced FCR by 9·79% in broilers. The result supports the use of B. subtilis CH16 of chicken intestinal origin as a feed supplement that promote weight gain in broilers. Significance and impact of the study: This study reports screening of Bacillus strains isolated from chicken gastrointestinal tracts for development of a feed supplement that promote weight gain in broilers. Of the seven Bacillus isolates with high sporulation efficiency (≥90%), Bacillus subtilis CH16 strain showed the best growth and biofilm formation at body temperature of broilers (42°C). In large-scale trials in broilers (n ≥ 1150 per group), CH16 spores induced a 9·17% increase in daily weight gain (ADG) and a 9·79% reduction in FCR while the commercial BioPlus(®) YC induced only a 5·89% increase in ADG and a 3·88% reduction in FCR.
摘要：
从鸡胃肠道中分离出孢子形成细菌菌株，以开发一种热稳定的饲料补充剂，可促进肉鸡体重增加。从分离物中筛选出7株孢子形成率超过90％的芽孢杆菌菌株，并鉴定出与枯草芽孢杆菌和地衣芽孢杆菌密切相关。在七种菌株中，选择枯草芽孢杆菌CH16来开发肉鸡的饲料补充剂，因为它形成了100%热稳定的孢子，在42°C下迅速生长并迅速形成生物膜。在肉鸡的大规模试验中(每组n≥1150)，饲喂CH16（3×10（6）CFUg（-1）颗粒）的组显示出较高的平均日增重（ADG=61·16）和较低的食物转化率（FCR=1·696）。饲喂枯草芽孢杆菌HU58（ADG=51·90，FCR=1·868），BioPlus组（ADG=59·32，FCR=1·807）和对照组（ADG=56·02，FCR=1·880）。总之,在肉鸡中，CH16孢子显着增加ADG9·17％，降低FCR9·79％。结果支持使用鸡肠来源的枯草芽孢杆菌CH16作为促进肉鸡体重增加的饲料补充剂。研究的意义和影响:本研究报道了从鸡胃肠道分离的芽孢杆菌菌株的筛选,用于开发促进肉鸡体重增加的饲料补充剂。7株芽孢杆菌产孢效率高(≥90%),枯草芽孢杆菌CH16菌株在肉鸡体温（42°C）下表现出最佳的生长和生物膜形成。在肉鸡的大规模试验中(每组n≥1150)，CH16孢子导致每日体重增加（ADG）增加9·17％，FCR减少9·79％，而商业BioPlus（®）YC仅导致ADG增加5·89％，FCR减少3·88％。
