具有增强的抗肿瘤效力的抗表皮生长因子受体的靶选择性活化抗体的产生和表征。
关键词: ADCC ADCC, antibody-dependent cell-mediated cytotoxicity CCK-8, Cell Counting Kit 8Yun CI, confidence interval CRC, colorectal cancer ECD, extracellular domain EGFR EGFR VIII, EGFR Type III Variant EGFR, epidermal growth factor receptor ELISA, enzyme-linked immunosorbent assay HC, heavy chain IgG, Immunoglobulin G LC, light chain Probody™ SEC, size exclusion chromatography SPR, surface plasmon resonance TKI, tyrosine kinase inhibitor mAb, monoclonal antibody monoclonal antibody panitumumab target-selective activation uPA, urokinase-type plasminogen activator
Mesh : Animals Antibodies, Monoclonal / genetics immunology pharmacology Antibodies, Neoplasm / genetics immunology pharmacology CHO Cells Cell Line, Tumor Colorectal Neoplasms / drug therapy immunology pathology Cricetinae Cricetulus ErbB Receptors / antagonists & inhibitors genetics immunology Female Humans Immunoglobulin Variable Region / genetics immunology pharmacology Mice Mice, Inbred BALB C Mice, Nude Panitumumab Protein Engineering Xenograft Model Antitumor Assays
来 源:
DOI:10.1080/19420862.2015.1008352
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