Abstract：
To elicit potent humoral immunity and produce adequate neutralizing antibody especially in the genital tract and eventually to promote its immunogenicity, we designed an Eppin B-cell-dominant-epitope-based mimovirus vaccine with an RGD motif which can be nasally inoculated into male mice. Our results indicate that this immune strategy successfully generated a high antibody response with significantly higher anti-Eppin IgA in the genital tract, and eventually achieve significant inhibition of fertility without any interference with testis function and alteration in structural integrity. The fertility rate of the females mating with the vaccinated males declined and the progeny size was greatly reduced, but the contraceptive efficacy was still far from that of immunocontraceptives for human use. However, the research showed a new contraceptive vaccine construction and inoculation avenue, that is, mimovirus vaccine delivered nasally. Further investigation geared toward improving fertility inhibition efficacy using this inoculation strategy still remains to be explored.
摘要：
引起有效的体液免疫，特别是在生殖道产生足够的中和抗体，并最终促进其免疫原性，我们设计了一种基于EppinB细胞显性表位的模拟病毒疫苗，该疫苗具有RGD基序，可通过鼻接种到雄性小鼠中。我们的结果表明，这种免疫策略成功地产生了高抗体反应，在生殖道中具有明显更高的抗EppinIgA，并最终实现对生育力的显着抑制，而不会干扰睾丸功能和结构完整性的改变。与接种疫苗的雄性交配的雌性的生育率下降，后代的大小大大减少，但是避孕效果与人类使用的免疫避孕药还相差甚远。然而,这项研究表明了一种新的避孕疫苗的构建和接种途径，也就是说,模拟病毒疫苗经鼻递送。使用这种接种策略改善生育力抑制功效的进一步研究仍有待探索。
