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Abstract：
Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic and phenotypic, are among the problems that continue to affect cell culture. Many of these problems are avoidable with the necessary foresight, and these Guidelines have been prepared to provide those new to the field and others engaged in teaching and instruction with the information necessary to increase their awareness of the problems and to enable them to deal with them effectively. The Guidelines cover areas such as development, acquisition, authentication, cryopreservation, transfer of cell lines between laboratories, microbial contamination, characterisation, instability and misidentification. Advice is also given on complying with current legal and ethical requirements when deriving cell lines from human and animal tissues, the selection and maintenance of equipment and how to deal with problems that may arise.
摘要：
细胞系错误识别和微生物污染，如支原体，加上不稳定，遗传和表型，是继续影响细胞培养的问题之一。这些问题中有许多是可以避免的，只要有必要的远见，这些准则是为了向那些新的领域和从事教学和指导的其他人提供必要的信息，以提高他们对问题的认识，并使他们能够有效地处理这些问题。准则涵盖发展、收购,身份验证,冷冻保存,实验室之间的细胞系转移，微生物污染，表征,不稳定和错误识别。在从人类和动物组织中提取细胞系时，还提供了有关遵守当前法律和道德要求的建议，设备的选择和维护，以及如何处理可能出现的问题。
