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Abstract：
BACKGROUND: We aim to find a storage protocol for vessels that preserves their dimensional, histologic, and mechanical characteristics to facilitate reproducible anastomosis experiments and microsurgical training with constant quality.
METHODS: We compared stored rabbit aortas, harvested in a slaughterhouse, using five different protocols with fresh controls. Aortas were preserved for 125 d in (1) NaCl 0.9% at -18°C, (2) Roswell Park Memorial Institute 1640 90% with 10% dimethyl sulfoxide (RPMI/DMSO) at -18°C, (3) RPMI/DMSO at -70°C, (4) glycerol 85% at 4°C, and (5) glycerol in stepwise increased concentrations until 85% at 4°C. After preservation, we measured vessel diameter, wall thickness, and Young\'s Modulus indicating stiffness. Neurosurgeons compared stored vessels with fresh vessels, blinded for preservation subgroup. We performed histologic assessment blinded for preservation subgroup.
RESULTS: Fresh rabbit aortas showed a mean diameter of 2.65 ± 0.14 mm, a mean wall thickness of 126 ± 22 μm, and a Young\'s Modulus of 11.4 ± 2.4 N/mm(2). NaCl 0.9%-preserved aortas showed a significantly increased vessel diameter and decreased stiffness. RPMI/DMSO-preserved aortas showed no significant differences from fresh aortas in dimensions and mechanical characteristics. Glycerol-preserved tissue showed a significant increase in wall thickness, a related significant decrease in diameter, and increase in stiffness. Neurosurgeons regarded RPMI/DMSO tissue as most comparable with fresh tissue. Histologic assessment revealed no differences between the different protocols and fresh control group.
CONCLUSIONS: Storage of rabbit aortas in RPMI/DMSO most adequately preserves their dimensional and mechanical properties.
METHODS: We compared stored rabbit aortas, harvested in a slaughterhouse, using five different protocols with fresh controls. Aortas were preserved for 125 d in (1) NaCl 0.9% at -18°C, (2) Roswell Park Memorial Institute 1640 90% with 10% dimethyl sulfoxide (RPMI/DMSO) at -18°C, (3) RPMI/DMSO at -70°C, (4) glycerol 85% at 4°C, and (5) glycerol in stepwise increased concentrations until 85% at 4°C. After preservation, we measured vessel diameter, wall thickness, and Young\'s Modulus indicating stiffness. Neurosurgeons compared stored vessels with fresh vessels, blinded for preservation subgroup. We performed histologic assessment blinded for preservation subgroup.
RESULTS: Fresh rabbit aortas showed a mean diameter of 2.65 ± 0.14 mm, a mean wall thickness of 126 ± 22 μm, and a Young\'s Modulus of 11.4 ± 2.4 N/mm(2). NaCl 0.9%-preserved aortas showed a significantly increased vessel diameter and decreased stiffness. RPMI/DMSO-preserved aortas showed no significant differences from fresh aortas in dimensions and mechanical characteristics. Glycerol-preserved tissue showed a significant increase in wall thickness, a related significant decrease in diameter, and increase in stiffness. Neurosurgeons regarded RPMI/DMSO tissue as most comparable with fresh tissue. Histologic assessment revealed no differences between the different protocols and fresh control group.
CONCLUSIONS: Storage of rabbit aortas in RPMI/DMSO most adequately preserves their dimensional and mechanical properties.
摘要：
背景：我们的目标是找到一种保留其尺寸的容器的存储协议，组织学，和机械特性，以促进可重复的吻合实验和显微手术训练，以恒定的质量。
方法：我们比较了储存的兔主动脉，在屠宰场收获,使用五种不同的方案和新的对照。主动脉在-18℃(1)0.9%NaCl中保存125天，（2）罗斯威尔公园纪念研究所164090％，在-18°C下使用10％二甲基亚砜（RPMI/DMSO），（3）RPMI/DMSO在-70°C，(4)甘油85%，4℃，和(5)在4°C下逐步增加浓度直至85%的甘油。保存后,我们测量了血管直径，壁厚,和杨氏模量表示刚度。神经外科医生将储存的血管与新鲜血管进行了比较，保留亚组盲化。我们对保留亚组进行了盲化的组织学评估。
结果：新鲜兔主动脉的平均直径为2.65±0.14mm，平均壁厚为126±22μm，杨氏模量为11.4±2.4N/mm(2)。0.9％的NaCl保存的主动脉显示出血管直径显着增加，刚度降低。RPMI/DMSO保存的主动脉在尺寸和机械特性上与新鲜主动脉没有显着差异。甘油保存的组织显示壁厚显著增加，相关的直径显著减小，并增加刚度。神经外科医生认为RPMI/DMSO组织与新鲜组织最具可比性。组织学评估显示不同方案与新鲜对照组之间没有差异。
结论：在RPMI/DMSO中储存兔主动脉最充分地保留了它们的尺寸和机械特性。
方法：我们比较了储存的兔主动脉，在屠宰场收获,使用五种不同的方案和新的对照。主动脉在-18℃(1)0.9%NaCl中保存125天，（2）罗斯威尔公园纪念研究所164090％，在-18°C下使用10％二甲基亚砜（RPMI/DMSO），（3）RPMI/DMSO在-70°C，(4)甘油85%，4℃，和(5)在4°C下逐步增加浓度直至85%的甘油。保存后,我们测量了血管直径，壁厚,和杨氏模量表示刚度。神经外科医生将储存的血管与新鲜血管进行了比较，保留亚组盲化。我们对保留亚组进行了盲化的组织学评估。
结果：新鲜兔主动脉的平均直径为2.65±0.14mm，平均壁厚为126±22μm，杨氏模量为11.4±2.4N/mm(2)。0.9％的NaCl保存的主动脉显示出血管直径显着增加，刚度降低。RPMI/DMSO保存的主动脉在尺寸和机械特性上与新鲜主动脉没有显着差异。甘油保存的组织显示壁厚显著增加，相关的直径显著减小，并增加刚度。神经外科医生认为RPMI/DMSO组织与新鲜组织最具可比性。组织学评估显示不同方案与新鲜对照组之间没有差异。
结论：在RPMI/DMSO中储存兔主动脉最充分地保留了它们的尺寸和机械特性。
