METHODS: A 35-year-old woman with history of IVF-ET treatment failure was deemed eligible for an ICSI procedure because of the male factor. Ovarian stimulation was performed according to the agonist long protocol. Eight MII oocytes were fertilized and seven embryos were obtained. Continuous embryo monitoring was performed with the use of Primo Vision system. Forty-four hours after fertilization only 2 correctly developing embryos were identified. They were transferred on day 3. The development of the remaining 5 embryos was arrested. These embryos did not achieve the blastocyst stage on day 5-6 after fertilization. Forty days after embryo transfer a twin pregnancy, confirmed with fetal heart rate of both fetuses, was revealed on ultrasound examination. Currently the patient is at 27 weeks of ongoing twin gestation.
CONCLUSIONS: The system of continuous embryo monitoring introduces new criteria for the examination of embryo development. These new parameters can be useful in clinical practice. However prospective randomized studies are necessary to provide data confirming the usefulness of time-lapse technique in IVF treatment.
方法:由于男性因素,一名有IVF-ET治疗失败史的35岁女性被认为有资格进行ICSI手术。根据长激动剂方案进行卵巢刺激。使8个MII卵母细胞受精并获得7个胚胎。使用PrimoVision系统进行持续胚胎监测。受精后44小时,仅鉴定出2个正确发育的胚胎。他们在第三天被转移。其余5个胚胎的发育被阻止。这些胚胎在受精后第5-6天没有达到胚泡阶段。胚胎移植后四十天,双胎妊娠,证实了两个胎儿的胎儿心率,在超声检查中发现。目前,患者正在进行27周的双胎妊娠。
结论:连续胚胎监测系统为检查胚胎发育引入了新的标准。这些新参数可用于临床实践。然而,前瞻性随机研究是必要的,以提供数据证实延时技术在IVF治疗中的有用性。