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Abstract：
The transcriptional strategy of orchid fleck virus (OFV), which has a two-segmented negative-strand RNA genome and resembles plant nucleorhabdoviruses, remains unexplored. In this study, the transcripts of six genes encoded by OFV RNA1 and RNA2 in the poly(A)-enriched RNA fraction from infected plants were molecularly characterized. All of the OFV mRNAs were initiated at a start sequence 3\'-UU-5\' with one to three non-viral adenine nucleotides which were added at the 5\' end of each mRNA, whereas their 3\' termini ended with a 5\'-AUUUAAA(U/G)AAAA(A)n-3\' sequence. We also identified the presence of polyadenylated short transcripts derived from the 3\'-terminal leader regions of both genomic and antigenomic strands, providing the first example of plus- and minus-strand leader RNAs in a segmented minus-strand RNA virus. The similarity in the transcriptional strategy between this bipartite OFV and monopartite rhabdoviruses, especially nucleorhabdoviruses (family Rhabdoviridae) is additional support for their close relationship.
摘要：
兰花斑点病毒（OFV）的转录策略，它有一个两段的负链RNA基因组，类似于植物核病毒，仍未探索。在这项研究中,对来自受感染植物的富含poly（A）的RNA部分中OFVRNA1和RNA2编码的六个基因的转录本进行了分子表征。所有OFVmRNAs都在起始序列3'-UU-5'处启动，并在每个mRNA的5'末端添加一到三个非病毒腺嘌呤核苷酸，而他们的3'末端以5'-AUUAAA（U/G）AAAA（A）n-3'序列结束。我们还鉴定了来自基因组和反基因组链的3'末端前导区的聚腺苷酸化短转录物的存在。提供了分段负链RNA病毒中正链和负链前导RNA的第一个例子。这种二分OFV和单分弹状病毒之间的转录策略相似，特别是核病毒（弹状病毒科）是它们亲密关系的额外支持。
