Abstract：
BACKGROUND: Several studies indicate that changes in cerebrospinal fluid (CSF) composition depend on the disease stage and reflect modification of brain energy metabolism (BEM). Also, it has been reported that a decline in cognitive functions may be mitigated by incorporating nutraceuticals in the diet.
OBJECTIVE: Assuming the beneficial effect of nutraceuticals on BEM and oxidative damage, the aim of this study was to determine if the administration of a nutraceutical compound results in changes of select CSF biomarkers in healthy adult Beagle dogs.
METHODS: Two separate CSF and blood samples were obtained from 11 healthy adult Beagle dogs, before and after 50 days of treatment with a veterinary combined nutraceutical. CSF analysis included a total nucleated cell count, total protein, glucose, sodium, chloride, potassium, pyruvate, and lactate concentrations, and calculation of lactate/pyruvate ratio. CBC and serum biochemistry were also performed. The Wilcoxon test was used to analyze the significance of the changes after nutraceutical treatment.
RESULTS: All studied variables remained within reference intervals, before and after treatment. A significant increase in CSF sodium and glucose concentration, and a decrease in lactate levels, was observed after treatment (P < .05), and the lactate/pyruvate ratio was decreased after treatment (P = .05). In serum, sodium and chloride concentrations were significantly increased (P < .05), and creatinine concentration was significantly decreased (P < .05) after treatment.
CONCLUSIONS: After 50 days of treatment with a nutraceutical compound, CSF glucose, sodium, and lactate concentrations, and L/P ratio were significantly different, suggesting an influence of nutraceuticals\' administration on CSF composition.
OBJECTIVE: Assuming the beneficial effect of nutraceuticals on BEM and oxidative damage, the aim of this study was to determine if the administration of a nutraceutical compound results in changes of select CSF biomarkers in healthy adult Beagle dogs.
METHODS: Two separate CSF and blood samples were obtained from 11 healthy adult Beagle dogs, before and after 50 days of treatment with a veterinary combined nutraceutical. CSF analysis included a total nucleated cell count, total protein, glucose, sodium, chloride, potassium, pyruvate, and lactate concentrations, and calculation of lactate/pyruvate ratio. CBC and serum biochemistry were also performed. The Wilcoxon test was used to analyze the significance of the changes after nutraceutical treatment.
RESULTS: All studied variables remained within reference intervals, before and after treatment. A significant increase in CSF sodium and glucose concentration, and a decrease in lactate levels, was observed after treatment (P < .05), and the lactate/pyruvate ratio was decreased after treatment (P = .05). In serum, sodium and chloride concentrations were significantly increased (P < .05), and creatinine concentration was significantly decreased (P < .05) after treatment.
CONCLUSIONS: After 50 days of treatment with a nutraceutical compound, CSF glucose, sodium, and lactate concentrations, and L/P ratio were significantly different, suggesting an influence of nutraceuticals\' administration on CSF composition.
摘要：
背景：多项研究表明，脑脊液（CSF）成分的变化取决于疾病阶段，并反映了脑能量代谢（BEM）的改变。此外，据报道,通过在饮食中加入营养品,可以缓解认知功能的下降.
目的：假设营养品对BEM和氧化损伤的有益作用，这项研究的目的是确定在健康的成年比格犬中施用营养化合物是否导致选定的CSF生物标志物的变化。
方法：从11只健康的成年比格犬获得两个单独的CSF和血液样本，在用兽医联合营养品治疗50天之前和之后。CSF分析包括有核细胞总数，总蛋白质，葡萄糖，钠,氯化物,钾，丙酮酸,和乳酸浓度，并计算乳酸/丙酮酸比。还进行了CBC和血清生物化学。使用Wilcoxon检验分析营养治疗后变化的意义。
结果：所有研究变量均保持在参考区间内，治疗前后。脑脊液钠和葡萄糖浓度显著增加，乳酸水平下降，治疗后观察到（P<0.05），治疗后乳酸/丙酮酸比值下降（P=0.05）。在血清中，钠和氯浓度显著增加（P<0.05），治疗后肌酐浓度明显下降(P<0.05)。
结论：用营养化合物治疗50天后，CSF葡萄糖，钠,和乳酸浓度，和L/P比有显著差异，提示营养品给药对脑脊液成分的影响。
目的：假设营养品对BEM和氧化损伤的有益作用，这项研究的目的是确定在健康的成年比格犬中施用营养化合物是否导致选定的CSF生物标志物的变化。
方法：从11只健康的成年比格犬获得两个单独的CSF和血液样本，在用兽医联合营养品治疗50天之前和之后。CSF分析包括有核细胞总数，总蛋白质，葡萄糖，钠,氯化物,钾，丙酮酸,和乳酸浓度，并计算乳酸/丙酮酸比。还进行了CBC和血清生物化学。使用Wilcoxon检验分析营养治疗后变化的意义。
结果：所有研究变量均保持在参考区间内，治疗前后。脑脊液钠和葡萄糖浓度显著增加，乳酸水平下降，治疗后观察到（P<0.05），治疗后乳酸/丙酮酸比值下降（P=0.05）。在血清中，钠和氯浓度显著增加（P<0.05），治疗后肌酐浓度明显下降(P<0.05)。
结论：用营养化合物治疗50天后，CSF葡萄糖，钠,和乳酸浓度，和L/P比有显著差异，提示营养品给药对脑脊液成分的影响。
