关键词： AAO APX AsA Ascorbate recycling processes Ascorbic acid Ascorbic acid biosynthesis DHAR GDH GDP-l-galactose phosphorylase GDP-mannose pyrophosphorylase (mannose-1-phosphateguanyltransferase) GDP-mannose-3′, 5′-epimerase GGP GLDH GME GMP GPP GR MDHAR MI Non-heading Chinese cabbage PMI PMM T-AsA ascorbate oxidase ascorbate peroxidase ascorbic acid (vitamin C) d-GalUA d-Glu d-GluA d-Mannose/l-galactose pathway d-galacturonic acid d-glucose d-glucuronic acid d-mannose-6-phosphate isomerase dehydroascorbate reductase glutathione reductase l-Gal l-GalL l-GulL l-galactono-1,4-lactone l-galactono-1,4-lactone dehydrogenase l-galactose l-galactose dehydrogenase l-galactose-1-phosphate phosphatase l-gulono-1,4-lactone monodehydroascorbate reductase myo-inositol phosphomannomutase qRT-PCR quantitative real-time PCR total AsA

Mesh ： Antioxidants / metabolism Ascorbic Acid / biosynthesis genetics Brassica / enzymology genetics metabolism China Diet Galactose / metabolism Gene Expression Genes, Plant Humans Mannose / metabolism Plant Leaves Plant Proteins / genetics metabolism Plant Roots Plant Structures / metabolism Species Specificity

来  源：   DOI：10.1016/j.plaphy.2013.10.005   PDF(Sci-hub)

Abstract：
Ascorbic acid (L-AsA) is an important antioxidant in plants and humans. Vegetables are one of the main sources of ascorbic acid for humans. For instance, non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) is considered as one of the most important vegetables in south China. To elucidate the mechanism by which AsA accumulates, we systematically investigated the expression profiles of D-mannose/L-galactose pathway-related genes. We also investigated the recycling-related genes and AsA contents in different tissues of three non-heading Chinese cabbage cultivars, \'Suzhouqing\', \'Wutacai\' and \'Erqing\' containing different amounts of AsA. Our results showed that six genes [D-mannose-6-phosphate isomerase 1 (PMI1), GDP-L-galactose phosphorylase 1 (GGP1), GGP2, GGP4, GDP-mannose-3\', 5\'-epimerase1 (GME1), and GME2] were expressed at high level and ascorbate oxidase (AAO) was expressed at low level. This expression pattern contributes, at least partially, to higher AsA accumulation in the leaves and petioles than in the roots. Eight genes (PMI1, GME, GGP, L-galactose-1-phosphate phosphatase, L-galactose dehydrogenase, L-galactono-1, 4-lactone dehydrogenase, monodehydroascorbate reductase 1, and glutathione reductase1) were also expressed at high level; AAO and ascorbate peroxidase (APX) were expressed at low level. This expression pattern may similarly contribute to higher AsA accumulation in \'Wutacai\' and \'Suzhouqing\' than in \'Erqing\'. Therefore, the high expression levels of PMI, GME, and GGP and the low expression level of AAO contributed to the high AsA accumulation in non-heading Chinese cabbage.
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