Abstract：
Hypertension remains the main risk factor for cardiovascular death. Environmental and biological factors are known to contribute to the condition, and circulating creatine kinase was reported to be the main predictor of blood pressure in the general population. This was proposed to be because of high resistance artery creatine kinase-BB rapidly regenerating ATP for vascular contractility. Therefore, we assessed whether creatine kinase isoenzyme mRNA levels in human resistance arteries are associated with blood pressure. We isolated resistance-sized arteries from omental fat donated by consecutive women undergoing uterine fibroid surgery. Blood pressure was measured in the sitting position. Vessels of 13 women were included, 6 normotensive and 7 hypertensive, mean age 42.9 years (SE, 1.6) and mean systolic/diastolic blood pressure, 144.8 (8.0)/86.5 (4.3) mm Hg. Arteriolar creatine kinase isoenzyme mRNA was assessed using quantitative real-time polymerase chain reaction. Normalized creatine kinase B mRNA copy numbers, ranging from 5.2 to 24.4 (mean, 15.0; SE, 1.9), showed a near-perfect correlation with diastolic blood pressure (correlation coefficient, 0.9; 95% confidence interval, 0.6-1.0) and were well correlated with systolic blood pressure, with a 90% relative increase in resistance artery creatine kinase B mRNA in hypertensives compared with normotensives, normalized copy numbers were, respectively, 19.3 (SE, 2.0) versus 10.1 (SE, 2.1), P=0.0045. To our knowledge, this is the first direct evidence suggesting that resistance artery creatine kinase mRNA expression levels concur with blood pressure levels, almost doubling with hypertension. These findings add to the evidence that creatine kinase might be involved in the vasculature\'s pressor responses.
摘要：
高血压仍然是心血管死亡的主要危险因素。已知环境和生物因素会导致这种情况，循环肌酸激酶是一般人群血压的主要预测因子。这被认为是由于高阻力动脉肌酸激酶-BB快速再生ATP用于血管收缩性。因此,我们评估了人体阻力动脉中肌酸激酶同工酶mRNA水平是否与血压相关。我们从连续接受子宫肌瘤手术的妇女捐赠的网膜脂肪中分离出阻力大小的动脉。以坐姿测量血压。包括13名妇女的船只，6血压正常，7高血压，平均年龄42.9岁(SE，1.6）和平均收缩压/舒张压，144.8（8.0）/86.5（4.3）mmHg。使用定量实时聚合酶链反应评估动脉型肌酸激酶同工酶mRNA。标准化肌酸激酶BmRNA拷贝数，范围从5.2到24.4(平均值，15.0；SE，1.9),显示与舒张压接近完美的相关性(相关系数，0.9；95%置信区间，0.6-1.0），并且与收缩压密切相关，与正常高血压相比，高血压患者的阻力动脉肌酸激酶BmRNA相对增加90％，归一化拷贝数为，分别,19.3(SE,2.0）与10.1（SE，2.1),P=0.0045。据我们所知,这是第一个直接证据表明阻力动脉肌酸激酶mRNA表达水平与血压水平一致，高血压几乎翻了一番。这些发现增加了肌酸激酶可能参与脉管系统的升压反应的证据。
