Generation of induced pluripotent stem cells with high efficiency from human umbilical cord blood mononuclear cells.

• 影响指数: 6.409
• 发表时间: Oct 2013
• 来源期刊: Genomics Proteomics Bioinformatics
• DOI: 10.1016/j.gpb.2013.08.002
• PMID: 24080286
• 文章类型: Journal Article
• 中科院分区: Q3
• 方向: 生物
• 作者列表: Wang J,Gu Q,Hao J,Bai D,Liu L,Zhao X,Liu Z,Wang L,Zhou Q

关键词: Dox-inducible lentiviral system; Human UCBMCs; Human iPSCs

Mesh: Biomarkers / metabolism; Cell Culture Techniques / methods; Cell Differentiation; Cell Separation / methods; Fetal Blood / cytology metabolism; Germ Layers / cytology metabolism; Humans; Induced Pluripotent Stem Cells / cytology metabolism; Infant, Newborn; Lentivirus / genetics

Abstract

Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs (UCB-iPSCs) have characteristics that are identical to pluripotent human embryonic stem cells (hESCs). This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.