Abstract：
OBJECTIVE: To review the use of ophthalmic rose bengal to assess the ocular surface, especially considering the grading scales used.
METHODS: A literature search was undertaken to identify reports where either rose bengal or lissamine green had been used as a \'vital\' stain, with a special interest in identifying studies that provided data before and after treatment for dry eye and also considered the mechanism of action of these two chemicals.
RESULTS: Between 1985 and 2006, numerous clinical studies used a consistent grading scheme (that attributed to Van Bijsterveld) to assess the outcome of artificial tear treatments on dry eye patients. With such consistency, including the adoption of a treatment period of 1 month, comparisons can be made to indicate the efficacy of rose bengal staining to assess reduction in ocular surface desiccation. However, in the following years, several alternative grading schemes have been used for both rose bengal and lissamine green and assessment periods have been variable so making inter-study comparisons considerably more difficult to undertake. An attribute of rose bengal appears to be its ability to stain the nuclei of cells, but whether this also occurs for lissamine green is unclear.
CONCLUSIONS: Ophthalmic rose bengal has been successfully adopted for use to assess the ocular surface over many years as a vital stain. More research is needed to assess whether lissamine green ocular surface staining can simply be substituted for rose bengal in evaluation of dry eye treatments.
METHODS: A literature search was undertaken to identify reports where either rose bengal or lissamine green had been used as a \'vital\' stain, with a special interest in identifying studies that provided data before and after treatment for dry eye and also considered the mechanism of action of these two chemicals.
RESULTS: Between 1985 and 2006, numerous clinical studies used a consistent grading scheme (that attributed to Van Bijsterveld) to assess the outcome of artificial tear treatments on dry eye patients. With such consistency, including the adoption of a treatment period of 1 month, comparisons can be made to indicate the efficacy of rose bengal staining to assess reduction in ocular surface desiccation. However, in the following years, several alternative grading schemes have been used for both rose bengal and lissamine green and assessment periods have been variable so making inter-study comparisons considerably more difficult to undertake. An attribute of rose bengal appears to be its ability to stain the nuclei of cells, but whether this also occurs for lissamine green is unclear.
CONCLUSIONS: Ophthalmic rose bengal has been successfully adopted for use to assess the ocular surface over many years as a vital stain. More research is needed to assess whether lissamine green ocular surface staining can simply be substituted for rose bengal in evaluation of dry eye treatments.
摘要：
目的：回顾眼用玫瑰红评估眼表的应用，特别是考虑到使用的分级标准。
方法：进行了文献检索，以确定将玫瑰红或丽萨明绿用作“重要”染色的报告，对确定提供干眼治疗前后数据的研究特别感兴趣，并考虑了这两种化学物质的作用机制。
结果：在1985年至2006年之间，许多临床研究使用了一致的分级方案（归因于VanBijsterveld）来评估人工泪液治疗对干眼患者的影响。有了这样的一致性,包括采用1个月的治疗期，可以进行比较以表明玫瑰红染色的功效，以评估眼表干燥的减少。然而,在接下来的几年里，玫瑰孟加拉和lissaminegreen都使用了几种替代分级方案，并且评估期是可变的，因此使研究间的比较更加困难。玫瑰孟加拉的一个属性似乎是它能够染色细胞核，但这是否也发生在lissamine绿色尚不清楚。
结论：眼玫瑰红多年来已成功用于评估眼表作为一种重要的色斑。在评估干眼治疗的评估中，需要更多的研究来评估lissamine绿色眼表染色是否可以简单地替代玫瑰红。
方法：进行了文献检索，以确定将玫瑰红或丽萨明绿用作“重要”染色的报告，对确定提供干眼治疗前后数据的研究特别感兴趣，并考虑了这两种化学物质的作用机制。
结果：在1985年至2006年之间，许多临床研究使用了一致的分级方案（归因于VanBijsterveld）来评估人工泪液治疗对干眼患者的影响。有了这样的一致性,包括采用1个月的治疗期，可以进行比较以表明玫瑰红染色的功效，以评估眼表干燥的减少。然而,在接下来的几年里，玫瑰孟加拉和lissaminegreen都使用了几种替代分级方案，并且评估期是可变的，因此使研究间的比较更加困难。玫瑰孟加拉的一个属性似乎是它能够染色细胞核，但这是否也发生在lissamine绿色尚不清楚。
结论：眼玫瑰红多年来已成功用于评估眼表作为一种重要的色斑。在评估干眼治疗的评估中，需要更多的研究来评估lissamine绿色眼表染色是否可以简单地替代玫瑰红。
