关键代谢底物的可用性决定了癌细胞对线粒体解偶联的呼吸反应。
关键词: AMP-activated protein kinase AMPK Akt BPTES Cancer cell D-galactose D-glucose DMEM DMSO Dulbecco's Modified Eagle's medium ECA ETC Erk FBS FCCP GLS1 GLUT GSH Gal Glc Gln Glu Glutaminolysis Glycolysis HS L-glutamine MEFs Metabolic substrate Mitochondrial respiration NGF OCR OxPhos PMF PMPI Pi Pyr ROS RPMI Roswell Park Memorial Institute TMRM Uncoupling WM bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl)ethyl sulfide carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone dimethyl sulphoxide electron transport chain extracellular acidification fetal bovine serum glucose transporter glutamate glutathione horse serum iO(2) inorganic phosphate intracellular oxygen kidney-type glutaminase mitochondrial membrane potential mitochondrial proton gradient mitogen-activated protein kinase (MAPK) mouse embryonic fibroblasts nerve growth factor oxidative phosphorylation oxygen consumption rate plasma membrane potential plasma membrane potential indicator protein kinase B (PKB) proton motive force pyruvate reactive oxygen species tetramethyl rhodamine methyl ester working media ΔpH ΔΨm ΔΨp α-KG α-ketoglutarate
Mesh : Animals Apoptosis / genetics Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / chemistry metabolism Cell Respiration / physiology Energy Metabolism Galactose / metabolism Glucose / metabolism Glutamine / metabolism Glycolysis / genetics HCT116 Cells Humans Mice Mitochondria / metabolism Neoplasms / metabolism pathology Oxidative Phosphorylation Oxygen Consumption / physiology PC12 Cells Pyruvic Acid / metabolism Rats Substrate Specificity
来 源:
DOI:10.1016/j.bbabio.2013.07.008
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