OBJECTIVE: To determine the cut-off level for (Hb) A(2) when estimated by high performance liquid chromatography (HPLC) in the diagnosis of classical beta-thalassaemia trait, a condition in the homozygous state that results in beta-thalassaemia major and red blood cell transfusion dependency.
RESULTS: High performance liquid chromatography (HPLC) as a method for the measurement of (Hb) A(2) was rapid, and technically easy. A cut-off level of (Hb) A(2) >4.0 % predict the majority of carriers of classical beta-thalassaemia.
CONCLUSIONS: A full blood count (FBC), together with red blood cell indices generated on an automated blood counter in conjunction with the measurement of Hb A(2) on the VARIANT-BioRad, an automated HPLC machine and the beta-thal short program is an appropriate approach for the screening and presumptive identification of carriers of classical beta-thalassaemia prior to DNA studies for definitive diagnosis. In carriers for classical beta-thalassaemia, the MCV and MCH are <75 fl and <27 pg respectively with a Hb A(2) cut-off level > 4.0% [range 5.9 (4.5-8.1)] on the VARIANT-BioRad.
目的:为了确定通过高效液相色谱法(HPLC)估算的(Hb)A(2)在经典β-地中海贫血特征诊断中的截止水平,一种纯合子状态的疾病,导致β-地中海贫血和红细胞输血依赖。
结果:高效液相色谱(HPLC)作为测量(Hb)A(2)的方法是快速的,技术上容易。(Hb)A(2)>4.0%的截止水平预测了大多数典型β-地中海贫血的携带者。
结论:全血计数(FBC),连同在自动血液计数器上产生的红细胞指数,以及在VARIANT-BioRad上测量的HbA(2),在进行DNA研究以明确诊断之前,自动HPLC机器和β-thal短程序是筛选和推定鉴定经典β-地中海贫血携带者的适当方法.在典型β-地中海贫血的携带者中,MCV和MCH分别<75fl和<27pg,在VARIANT-BioRad上HbA(2)截止水平>4.0%[范围5.9(4.5-8.1)]。