Mesh : Animals Capillary Permeability Cell Membrane Permeability Cells, Cultured Diffusion Chambers, Culture Electric Impedance Endothelial Cells / cytology metabolism Endothelium, Vascular / cytology metabolism Evans Blue / analysis Intercellular Junctions / metabolism Kinetics Lung / cytology metabolism Mice Organ Culture Techniques / methods Organ Size Permeability Potentiometry / methods Pulmonary Edema / metabolism physiopathology Serum Albumin, Radio-Iodinated / analysis metabolism

来  源:   DOI:10.1007/978-1-61779-191-8_23

Abstract:
The methods for assessment of endothelial barrier permeability are vital tools of experimental biology. They allow us to measure permeability of endothelial monolayer in cell culture and in lung vessels or to determine formation of tissue edema resulting from increased permeability of vasculature. This chapter provides an overview of the most common protocols.
摘要:
评估内皮屏障通透性的方法是实验生物学的重要工具。它们使我们能够测量细胞培养物中和肺血管中内皮单层的通透性,或确定由脉管系统通透性增加引起的组织水肿的形成。本章概述了最常见的协议。
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