Interplay between the cpSRP pathway components, the substrate LHCP and the translocase Alb3: an in vivo and in vitro study.

• 影响指数: 3.864
• 发表时间: Oct 2010 8
• 来源期刊: FEBS Lett
• DOI: 10.1016/j.febslet.2010.08.053
• PMID: 20828566
• 文章类型: Journal Article
• 中科院分区: Q3
• 方向: 生物
• 作者列表: Bals T,Dünschede B,Funke S,Schünemann D

Mesh: Arabidopsis / genetics metabolism; Arabidopsis Proteins / chemistry genetics metabolism; Chloroplast Proteins; Chloroplasts / metabolism; Light-Harvesting Protein Complexes / chemistry genetics metabolism; Membrane Proteins / chemistry genetics metabolism; Multiprotein Complexes; Protein Interaction Domains and Motifs; Recombinant Fusion Proteins / chemistry genetics metabolism; Signal Recognition Particle / chemistry genetics metabolism; Signal Transduction; Thylakoids / metabolism

Abstract

The chloroplast signal recognition particle (cpSRP) and its receptor, cpFtsY, posttranslationally target the nuclear-encoded light-harvesting chlorophyll-binding proteins (LHCPs) to the translocase Alb3 in the thylakoid membrane. In this study, we analyzed the interplay between the cpSRP pathway components, the substrate protein LHCP and the translocase Alb3 by using in vivo and in vitro techniques. We propose that cpSRP43 is crucial for the binding of LHCP-loaded cpSRP and cpFtsY to Alb3. In addition, our data suggest that a direct interaction between Alb3 and LHCP contributes to the formation of this complex.