关键词: PCR-RFLP SAG2 Toxoplasma gondii stray cat

Mesh : Animals Antigens, Protozoan / genetics Blood / parasitology Cat Diseases / parasitology Cats Cluster Analysis DNA Fingerprinting / methods DNA, Protozoan / genetics isolation & purification Genotype Korea Polymerase Chain Reaction / methods Polymorphism, Restriction Fragment Length Protozoan Proteins / genetics Toxoplasma / classification genetics isolation & purification Toxoplasmosis, Animal / parasitology

来  源:   DOI:10.3347/kjp.2009.47.4.413   PDF(Pubmed)

Abstract:
Genotyping of Toxoplasma gondii has been performed in 23 PCR positive blood samples from stray cats in Korea. We used 2 separate PCR-restriction fragment length polymorphism (RFLP) patterns of SAG2 gene, amplifying the 5\' and 3\' ends of the locus. The results revealed that all samples belonged to the type I clonal lineage. Although T. gondii organisms were not isolated from the samples, the results of the present study represent that stray cats with T. gondii infection should be seriously concerned in our environment. Adequate and continuous control programs of stray cats are needed to reduce the risk of transmission of T. gondii as a zoonotic infection threatening the public health.
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