PDF(Sci-hub)
Abstract：
Previous studies have suggested that periodontal ligament stem cells (PDLSCs) play crucial role in regeneration of periodontal defects, and recently tissue engineering based on PDLSCs to enhance periodontal regeneration has been the focus of periodontal research. A theoretical way to achieve this goal would be to provide a \"stimulatory\'\' environment to rapidly expand PDLSCs in vitro to expedite tissue engineering of periodontium. We hypothesize that three-dimensional (3D) dynamic simulated microgravity (SMG) culture system have effect on periodontal stem cells, and would benefit periodontal stem cells proliferation and differentiation, but up to now, there are no related reports on this aspect. In this study, we investigated the biological effect of three-dimensional dynamic SMG induced by rotary cell culture system (RCCS) on human periodontal ligament stem cells (hPDLSCs) in vitro. hPDLSCs were isolated from surgically extracted human teeth and enriched by collecting multiple colonies. hPDLSCs were inoculated on Cytodex 3 microcarriers and cultured in RCCS. The results showed that SMG affected the biology of hPDLSCs as indicated by promotion of proliferation and viability, alterations of morphology, and disorganization of microfilament system. Besides, SMG-treated hPDLSCs presented increased matrix mineralization and up-regulated expression of mineralization associated genes after incubation in osteogenic medium. For it is the first time to investigate effects of SMG on PDLSCs, the research may lend insight into variations of cell response in 3D environment, and contribute to achievement of desirable periodontal regeneration utilizing PDLSCs-based tissue engineering approaches.
摘要：
以往的研究表明,牙周膜干细胞(PDLSCs)在牙周缺损的再生过程中起着至关重要的作用。近年来，基于PDLSCs的组织工程促进牙周再生已成为牙周研究的热点。实现这一目标的理论方法是提供一个“刺激性”环境，以在体外快速扩增PDLSCs，以加快牙周组织工程。我们假设三维（3D）动态模拟微重力（SMG）培养系统对牙周干细胞有影响，有利于牙周干细胞的增殖和分化，但到现在为止,这方面没有相关报道。在这项研究中,我们研究了旋转细胞培养系统（RCCS）诱导的三维动态SMG对人牙周膜干细胞（hPDLSCs）的生物学效应。从手术提取的人牙齿中分离hPDLSC,并通过收集多个集落来富集。将hPDLSC接种在Cytodex3微载体上并在RCCS中培养。结果表明,SMG对hPDLSCs的生物学特性有明显的影响,可以促进细胞的增殖和活力,形态学的改变，和微丝系统的混乱。此外,SMG处理的hPDLSCs在成骨培养基中孵育后，基质矿化增加，矿化相关基因的表达上调。这是第一次研究SMG对PDLSCs的影响，这项研究可能有助于深入了解3D环境中细胞反应的变化，并有助于利用基于PDLSCs的组织工程方法实现理想的牙周再生。
