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Abstract：
OBJECTIVE: To determine the meiotic segregation in large-headed, multiple-tailed spermatozoa.
METHODS: Analysis of sperm nuclei by fluorescence in situ hybridization (FISH).
METHODS: University hospital.
METHODS: A 34-year-old man with 100% morphologically abnormal spermatozoa.
METHODS: Dual-color FISH for chromosomes 13 and 21 and triple-color FISH for chromosomes X, Y, and 18 were performed.
METHODS: Aneuploidy rates.
RESULTS: More than 99% of the spermatozoa had abnormal content for chromosomes X, Y, 13, 18, and 21. Diploidy, triploidy, and tetraploidy rates were found to be 18.42%, 6.14%, and 33.99% in triple-color FISH and to be 16.09%, 16.28%, and 38.95% in dual-color FISH.
CONCLUSIONS: Our results and those from other investigators show that large-headed, multiple-tailed spermatozoa are associated with a high rate of polyploidy and aneuploidy. Intracytoplasmic sperm injection should not be recommended to those patients, not only because of its low success rate but also because of its high genetic risk.
METHODS: Analysis of sperm nuclei by fluorescence in situ hybridization (FISH).
METHODS: University hospital.
METHODS: A 34-year-old man with 100% morphologically abnormal spermatozoa.
METHODS: Dual-color FISH for chromosomes 13 and 21 and triple-color FISH for chromosomes X, Y, and 18 were performed.
METHODS: Aneuploidy rates.
RESULTS: More than 99% of the spermatozoa had abnormal content for chromosomes X, Y, 13, 18, and 21. Diploidy, triploidy, and tetraploidy rates were found to be 18.42%, 6.14%, and 33.99% in triple-color FISH and to be 16.09%, 16.28%, and 38.95% in dual-color FISH.
CONCLUSIONS: Our results and those from other investigators show that large-headed, multiple-tailed spermatozoa are associated with a high rate of polyploidy and aneuploidy. Intracytoplasmic sperm injection should not be recommended to those patients, not only because of its low success rate but also because of its high genetic risk.
摘要：
目的：为了确定大头减数分裂的分离，多尾精子。
方法：通过荧光原位杂交（FISH）分析精子核。
方法：大学医院。
方法：一名34岁男性，精子形态异常100%。
方法：染色体13和21的双色FISH和染色体X的三色FISH，Y,执行了18次。
方法：非整倍体率。
结果：超过99％的精子具有X染色体的异常含量，Y,13、18和21。二倍体,三倍体,四倍体率为18.42%，6.14%，三色FISH占33.99%，占16.09%，16.28%,和38.95％的双色FISH。
结论：我们和其他研究者的研究结果表明，大头，多尾精子与高多倍体和非整倍体率相关。卵胞浆内单精子注射不应推荐给这些患者，不仅因为它的成功率低，而且因为它的遗传风险高。
方法：通过荧光原位杂交（FISH）分析精子核。
方法：大学医院。
方法：一名34岁男性，精子形态异常100%。
方法：染色体13和21的双色FISH和染色体X的三色FISH，Y,执行了18次。
方法：非整倍体率。
结果：超过99％的精子具有X染色体的异常含量，Y,13、18和21。二倍体,三倍体,四倍体率为18.42%，6.14%，三色FISH占33.99%，占16.09%，16.28%,和38.95％的双色FISH。
结论：我们和其他研究者的研究结果表明，大头，多尾精子与高多倍体和非整倍体率相关。卵胞浆内单精子注射不应推荐给这些患者，不仅因为它的成功率低，而且因为它的遗传风险高。
