Abstract：
The appearance of new peaks in the 7.7-8.6 and 6.8-7.4 ppm regions of the postexercise (1)H spectrum of frog muscle is reported. These new peaks result from the splitting of single pre-exercise carnosine C-2 and C-4 peaks into two peaks, representing the intracellular pH (pH(I)) of oxidative and glycolytic fibers. The following data support this conclusion: 1) comparison of means and regression analysis indicates equivalence of the pH(I) measurements by (1)H and (31)P NMR; 2) the pre- and poststimulation concentrations of carnosine are equal; 3) in ischemic rat hindlimb muscles, the presence of a single, more acidic peak in the plantaris; a single, less acidic peak in the soleus; and two peaks (more and less acidic) in the gastrocnemius correspond to published values for the fiber-type composition of these muscles; and 4) in muscles treated with iodoacetate prior to and during stimulation, a second peak never appears. These data indicate that it is feasible to measure separately the pH(I) of oxidative and glycolytic fibers using (1)H NMR spectroscopy.
摘要：
据报道，在运动后（1）青蛙肌肉的H谱的7.7-8.6和6.8-7.4ppm区域出现了新峰。这些新的峰值是由单一的运动前肌肽C-2和C-4的峰值分裂成两个峰值，代表氧化和糖酵解纤维的细胞内pH（pH（I））。以下数据支持这一结论：1）均值比较和回归分析表明通过（1）H和（31）PNMR测量的pH（I）等效；2）肌肽的刺激前后浓度相等；3）在缺血性大鼠后肢肌肉中，一个人的存在，足底酸性峰；单个，比目鱼肌中的酸性峰较少；腓肠肌中的两个峰（或多或少的酸性）对应于这些肌肉的纤维型组成的公开值；和4）在刺激之前和期间用碘乙酸酯处理的肌肉中，第二个高峰永远不会出现。这些数据表明，使用（1）HNMR光谱分别测量氧化和糖酵解纤维的pH（I）是可行的。
