Mesh ： Animals Cercopithecinae DNA Primers DNA, Viral / analysis Gene Products, pol / genetics HIV Infections / virology HIV Integrase / genetics HIV-1 / classification genetics isolation & purification HIV-2 / classification genetics isolation & purification Humans Molecular Sequence Data Monkey Diseases / virology Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Simian Acquired Immunodeficiency Syndrome / virology Simian Immunodeficiency Virus / classification genetics isolation & purification

来  源：   DOI：10.1128/JCM.40.9.3167-3171.2002   PDF(Sci-hub)   PDF(Pubmed)

Abstract：
Human immunodeficiency virus type 2 (HIV-2) is the result of cross-species transmission of simian immunodeficiency virus (SIV) from sooty mangabey monkeys to humans. Primer pairs (intHIV-2/SIV) based on a region of integrase that has considerable homology across HIV-2 and SIV lineages were designed to develop a broadly cross-reactive molecular assay to detect lentivirus infection in primates. The intHIV-2/SIV primers detect HIV-2 and simian viruses SIVcpz, SIVsmm, SIVsyk, SIVagm, and SIVmnd. The primers are also capable of amplifying some HIV-1 strains. Additionally, sequences from the integrase amplicons were of sufficient genetic diversity to permit not only phylogenetic clustering of all simian viruses to their respective lineages but also HIV type and group classification. Thus, the primers described here provide a method to detect primate lentiviruses from diverse species of nonhuman primates, as well as from persons infected with HIV-1 and HIV-2.
摘要：
暂无翻译
公众号